Zobrazeno 1 - 10
of 1 554
pro vyhledávání: '"LIMITING DILUTION"'
Autor:
Chengchen Pang, Qiang Liu, Lin Chen, Bei Yuan, Chuanyun Zha, Kunying Nie, Haitao Xu, Keyun Ren, Chunlei Yu, Yemin Guo, Qingqing Yang
Publikováno v:
Toxins, Vol 16, Iss 1, p 11 (2023)
To achieve accurate detection of AFB1 toxin content in agricultural products and avoid false-positive rates in the assays, the specificity of mAbs is critical. We improved the specificity of the prepared monoclonal antibodies by modifying the traditi
Externí odkaz:
https://doaj.org/article/ae461ce865084f64935c7d4ea9437394
Publikováno v:
Viruses, Vol 15, Iss 11, p 2152 (2023)
Adenoviruses are promising vectors for vaccine production and gene therapy. Despite all the efforts in removing animal-derived components such as fetal bovine serum (FBS) during the production of adenovirus vector (AdV), FBS is still frequently emplo
Externí odkaz:
https://doaj.org/article/426ebe9e52fa45668ee24074b987af40
Publikováno v:
Malaria Journal, Vol 20, Iss 1, Pp 1-7 (2021)
Abstract Background Molecular and genetic studies of blood-stage Plasmodium falciparum parasites require limiting dilution cloning and prolonged cultivation in microplates. The entire process is laborious and subject to errors due to inaccurate dilut
Externí odkaz:
https://doaj.org/article/50c1c3a57b3643f39947d1fd07117f16
Autor:
Jonathan Hollmann, Johanna Brecht, Roman Goetzke, Julia Franzen, Anton Selich, Marco Schmidt, Monika Eipel, Alina Ostrowska, Jan Hapala, Eduardo Fernandez-Rebollo, Gerhard Müller-Newen, Michael Rothe, Thomas Eggermann, Martin Zenke, Wolfgang Wagner
Publikováno v:
Stem Cell Research & Therapy, Vol 11, Iss 1, Pp 1-13 (2020)
Abstract Background The use of mesenchymal stromal cells (MSCs) for research and clinical application is hampered by cellular heterogeneity and replicative senescence. Generation of MSC-like cells from induced pluripotent stem cells (iPSCs) may circu
Externí odkaz:
https://doaj.org/article/df179f3bd39144b6b52fc3d37e4a90fc
Publikováno v:
Asian Pacific Journal of Tropical Biomedicine, Vol 10, Iss 6, Pp 248-253 (2020)
Objective: To compare limiting dilution assay and real-time PCR methods in Leishmania tropica parasite load measurement in vaccinated mice. Methods: BALB/c mice were vaccinated by Leishmania tropica soluble Leishmania antigen or recombinant Leishmani
Externí odkaz:
https://doaj.org/article/91f71744af4446c7b30c88c21606690d
Autor:
Annabelle Walz, Maëlle Duffey, Ghaith Aljayyoussi, Sibylle Sax, Didier Leroy, Dominique Besson, Jeremy N. Burrows, Mohammed H. Cherkaoui-Rbati, Nathalie Gobeau, Marie-Anne Westwood, Christoph Siethoff, Francisco-Javier Gamo, Pascal Mäser, Sergio Wittlin
Publikováno v:
Pharmaceuticals, Vol 16, Iss 2, p 163 (2023)
With artemisinin-resistant Plasmodium falciparum parasites emerging in Africa, the need for new antimalarial chemotypes is persistently high. The ideal pharmacodynamic parameters of a candidate drug are a rapid onset of action and a fast rate of para
Externí odkaz:
https://doaj.org/article/ed61e47142b2471096b9a830abacea17
Autor:
Salma Fuadiyah, Kittipat Chotchindakun, Rungrueang Phatthanakun, Panwong Kuntanawat, Montarop Yamabhai
Publikováno v:
Micromachines, Vol 13, Iss 11, p 1939 (2022)
Genetically-modified monoclonal cell lines are currently used for monoclonal antibody (mAbs) production and drug development. The isolation of single transformed cells is the main hindrance in the generation of monoclonal lines. Although the conventi
Externí odkaz:
https://doaj.org/article/139e5548b7d4402d9bd25e44d14d5231
Publikováno v:
Parasites & Vectors, Vol 12, Iss 1, Pp 1-15 (2019)
Abstract Background Babesia bovis is the causal agent of Asiatic redwater, transmitted by the pandemic tick Rhipicephalus (Boophilus) microplus. Disease control may target the tick vector using acaricides or anti-tick vaccines, or the parasite using
Externí odkaz:
https://doaj.org/article/ccfdc1c14acc4962964f2b5bce357768
Autor:
Anna M. Ehlers, Constance F. den Hartog Jager, Tineke Kardol-Hoefnagel, Miriam M.D. Katsburg, André C. Knulst, Henny G. Otten
Publikováno v:
Frontiers in Immunology, Vol 12 (2021)
Human monoclonal antibodies (mAbs) are valuable tools to link genetic information with functional features and to provide a platform for conformational epitope mapping. Additionally, combined data on genetic and functional features provide a valuable
Externí odkaz:
https://doaj.org/article/3818383921aa42ef9034817c888a39d8
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