Zobrazeno 1 - 10
of 45
pro vyhledávání: '"L. V. Trilisenko"'
Publikováno v:
Canadian Journal of Microbiology. 67:226-230
Plasma membrane integrity is a key to cell viability. Currently, the main approach to assessing plasma membrane integrity is the detection of penetration of special dyes, such as trypan blue and propidium iodide, into the cells. However, this method
Autor:
Tatiana Kulakovskaya, Nadezhda A Andreeva, Michail Eldarov, L. P. Ryazanova, L. V. Trilisenko, Larisa Ledova
Publikováno v:
Biochemistry (Moscow). 86:S96-S108
Inorganic polyphosphates (polyP) are the linear polymers of orthophosphoric acid varying in the number of phosphate residues linked by the energy-rich phosphoanhydride bonds. PolyP is an essential component in living cells. Knowledge of polyP metabol
VTC4 Polyphosphate Polymerase Knockout Increases Stress Resistance of Saccharomyces cerevisiae Cells
Autor:
Ivan V. Kulakovskiy, E. V. Kulakovskaya, Alexey Yu. Fedorov, Tatiana Kulakovskaya, M. A. Eldarov, Alexander Tomashevsky, L. V. Trilisenko
Publikováno v:
Biology, Vol 10, Iss 487, p 487 (2021)
Biology
Volume 10
Issue 6
Biology
Volume 10
Issue 6
Simple Summary Inorganic polyphosphate, a linear polymer of orthophosphoric acid, plays an important role in microorganisms’ stress resistance. Vtc4 protein synthetizes inorganic polyphosphate in yeast. Here we show that yeast lacking this protein
Autor:
Alexander Tomashevsky, E. V. Kulakovskaya, Alexey Yu. Fedorov, Tatiana Kulakovskaya, L. V. Trilisenko, M. A. Eldarov
Inorganic polyphosphate (polyP) is an important factor of stress tolerance in microbial cells. In yeast, the major enzyme of polyP biosynthesis is Vtc4, a subunit of the vacuole transporter chaperone (VTC) complex. In this study, we demonstrated that
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52a7e7d444d5f466add5cddc8bd9b96b
https://doi.org/10.20944/preprints202010.0484.v1
https://doi.org/10.20944/preprints202010.0484.v1
Autor:
N. A. Andreeva, Anton Zvonarev, M. A. Eldarov, L. P. Ryazanova, Tatiana Kulakovskaya, L. V. Trilisenko
Publikováno v:
Applied Microbiology and Biotechnology. 102:5235-5244
Inorganic polyphosphate (polyP) is a significant regulatory and metabolic compound in yeast cells. We compared polyP content and localization, polyphosphatase activities, and transcriptional profile of polyP-related genes in industrially important me
Autor:
Tatiana Kulakovskaya, Airat Valiakhmetov, Vladimir Ostroumov, L. V. Trilisenko, Anton Zvonarev, Irina A. Eliseeva, Alexey A. Penin, Ivan V. Kulakovskiy
Publikováno v:
Cells
Volume 8
Issue 5
Cells, Vol 8, Iss 5, p 461 (2019)
Volume 8
Issue 5
Cells, Vol 8, Iss 5, p 461 (2019)
Inorganic polyphosphate (polyP) is crucial for adaptive reactions and stress response in microorganisms. A convenient model to study the role of polyP in yeast is the Saccharomyces cerevisiae strain CRN/PPN1 that overexpresses polyphosphatase Ppn1 wi
Publikováno v:
Advances in Enzyme Research. :144-151
The Saccharomyces cerevisiae polyphosphatase PPN1 (uniprot/Q04119) degrades inorganic polyphosphates both by cleaving Pi from the chain end and by fragmenting long-chain polymers into shorter ones. In this study, we have found a new activity of this
Publikováno v:
Journal of Bioscience and Bioengineering. 119:52-56
Inorganic polyphosphate performs many regulatory functions in living cells. The yeast exopolyphosphatase PPN1 is an enzyme with multiple cellular localization and probably variable functions. The Saccharomyces cerevisiae strain with overexpressed PPN
Publikováno v:
Journal of basic microbiology. 57(11)
The sensitivity to cadmium (Cd(II)), an important environmental pollutant, was studied in the cells of Saccharomyces cerevisiae strains with genetically altered polyphosphate metabolism. The strains overproducing polyphosphatases PPX1 or PPN1 were mo
Autor:
T. V. Kulakovskaya, L. V. Trilisenko
Publikováno v:
Biochemistry (Moscow). 79:478-482
Cells of the yeast Saccharomyces cerevisiae with a low content of polyphosphates (polyP) are characterized by disturbance of growth in medium with 0.5% glucose. The parent strain with polyP level reduced by phosphate starvation had a longer lag phase