Zobrazeno 1 - 10
of 209
pro vyhledávání: '"L. Schwartzbrod"'
Autor:
S. Monpoeho, A. Dehée, B. Mignotte, L. Schwartzbrod, V. Marechal, J.-C. Nicolas, S. Billaudel, V. Férré
Publikováno v:
BioTechniques, Vol 29, Iss 1, Pp 88-93 (2000)
We have developed a quantitative RTPCR method that can be used to determine the amount of enterovirus RNA in urban sludge samples. This method combines Taq-Man® technology with the ABI PrismTM 7700 real-time sequence detection system. We optimized a
Externí odkaz:
https://doaj.org/article/d83221de93164a0a96b9b278570b1528
Publikováno v:
Applied and Environmental Microbiology
Applied and Environmental Microbiology, American Society for Microbiology, 2003, 69 (7), pp.3965-3969. ⟨10.1128/AEM.69.7.3965-3969.2003⟩
Applied and Environmental Microbiology, American Society for Microbiology, 2003, 69 (7), pp.3965-3969. ⟨10.1128/AEM.69.7.3965-3969.2003⟩
Appropriate interpretation of a positive reverse transcription-PCR is an important issue for virus-related health hazard assessment because viral genomes and infectious viruses exhibit different behavior patterns in water. In this context, using Poli
Publikováno v:
Critical Reviews in Microbiology. 28:371-409
Many classes of pathogens excreted in feces are able to initiate waterborne infections. There are bacterial pathogens, including enteric and aquatic bacteria, enteric viruses, and enteric protozoa, which are strongly resistant in the water environmen
Autor:
A. Dehee, B. Mignotte, Sylviane Billaudel, J. C. Nicolas, S. Monpoeho, Virginie Ferré, V. Marechal, L. Schwartzbrod
Publikováno v:
BioTechniques. 29:88-93
We have developed a quantitative RTPCR method that can be used to determine the amount of enterovirus RNA in urban sludge samples. This method combines Taq-Man® technology with the ABI PrismTM 7700 real-time sequence detection system. We optimized a
Publikováno v:
Journal of Virological Methods. 77:17-26
When choosing an extraction method, two parameters have to be considered: recovery of the viral material and elimination or inactivation of inhibitory substances. Seven techniques for extracting hepatitis A virus (HAV) from stool and shellfish sample
Autor:
H. Kopecka, Jean-Marc Crance, Sylviane Billaudel, Christophe Gantzer, L. Schwartzbrod, Eric Dubois, Françoise S. Le Guyader, Monique Pommepuy
Publikováno v:
Oceanologica Acta (0399-1784) (Elsevier), 1998-11, Vol. 21, N. 6, P. 983-992
Oceanologica Acta
Oceanologica Acta, 1998, 21 (6), pp.983-992. ⟨10.1016/S0399-1784(99)80020-6⟩
Oceanologica Acta, Elsevier, 1998, 21 (6), pp.983-992. ⟨10.1016/S0399-1784(99)80020-6⟩
Oceanologica Acta
Oceanologica Acta, 1998, 21 (6), pp.983-992. ⟨10.1016/S0399-1784(99)80020-6⟩
Oceanologica Acta, Elsevier, 1998, 21 (6), pp.983-992. ⟨10.1016/S0399-1784(99)80020-6⟩
To improve the knowledge of the survival of enteric viruses in a marine environment, the influence of physico-chemical parameters (temperature, UV, salinity) on the survival of infectious poliovirus 1 and hepatitis A Virus (HAV) in seawater was first
Autor:
L. Schwartzbrod, Sylviane Billaudel, Berengere Mignotte, Armand Maul, Ousmane Traoré, Henri Laveran, Charlotte Arnal
Publikováno v:
Applied and Environmental Microbiology
Applied and Environmental Microbiology, American Society for Microbiology, 1998
Applied and Environmental Microbiology, American Society for Microbiology, 1998
Four methods of extraction and three methods of concentration of three enteric viruses from mussels were comparatively evaluated by reverse transcriptase PCR (RT-PCR). Shellfish were experimentally contaminated by immersion in seawater seeded with as
Publikováno v:
Canadian Journal of Microbiology
Canadian Journal of Microbiology, NRC Research Press, 1998, 44 (2), pp.190-194. ⟨10.1139/w97-134⟩
Canadian Journal of Microbiology, NRC Research Press, 1998, 44 (2), pp.190-194. ⟨10.1139/w97-134⟩
International audience; Après la chaleur, la conductivité semble être le deuxième meilleur facteur d'inactivation des virus dans les eaux de surface. Cette étude montre qu'avec le poliovirus-1 on obtient une relation linéaire très significativ
Publikováno v:
Marine Pollution Bulletin. 34:875-879
Four poliovirus extraction and three poliovirus concentration techniques designed for the study of mussel tissues were compared. The recovered infectious viral particles were confirmed by inoculation to cell cultures. The protocol that provided optim
Publikováno v:
Journal of Virological Methods
Journal of Virological Methods, Elsevier, 1997, 65 (2), pp.265-271. ⟨10.1016/S0166-0934(97)02193-9⟩
Journal of Virological Methods, Elsevier, 1997, 65 (2), pp.265-271. ⟨10.1016/S0166-0934(97)02193-9⟩
International audience; Standard methods for detecting enteroviruses in environmental samples require cell culture, which is time consuming and expensive. The reverse transcription-polymerase chain reaction (RT-PCR) is a rapid, sensitive method for d