Zobrazeno 1 - 10
of 14
pro vyhledávání: '"L. S. Izotova"'
Autor:
Natalya A. Mochulskaya, Alla Lapidus, Sergey V. Mashko, Andrey V. Mochulsky, Yury P. Vinetsky, Sergey A. Ketlinsky, Sergey V. Kotenko, L. S. Izotova, Lebedeva Mi, Debabov Vg, Vladimir P. Veiko
Publikováno v:
Gene. 97:259-266
A new dual-replicon recombinant plasmid, pPR53-tsr, has been constructed; it is a derivative of the expression vector pPR-TGATG-1 [Mashko et al., Gene 88 (1990) 121-126]. In contrast to its progenitor, pPR53-tsr is a low-copy-number (low-Cop) plasmid
Autor:
S V, Kotenko, L S, Izotova, O V, Mirochnitchenko, E, Esterova, H, Dickensheets, R P, Donnelly, S, Pestka
Publikováno v:
The Journal of biological chemistry. 276(4)
Interleukin-10 (IL-10)-related T cell-derived inducible factor (IL-TIF; provisionally designated IL-22) is a cytokine with limited homology to IL-10. We report here the identification of a functional IL-TIF receptor complex that consists of two recep
Autor:
I I, Shekhter, V P, Beĭko, M T, Bulenkov, O M, Khodova, M A, Kolevatykh, L S, Izotova, M I, Lebedeva, A Iu, Rudenskiĭ, S I, Borukhov, V I, Iurin
Publikováno v:
Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic]. 36(8)
The gene of human mutant (serine-17) fibroblast interferon was isolated with the use of highly efficient oligonucleotide-directed mutagenesis. On the basis of the constructed expression plasmid pPR-IFN Ser17 a strain producing human mutant beta-inter
Publikováno v:
Biomedical science. 2(4)
An epitope of human leukocyte alpha interferon A (IFN-A), which is recognized by the murine monoclonal antibody NK2, has been mapped by using four successive approaches. Limited proteolysis of the IFN-A chain, followed by electrophoresis, Western blo
Autor:
S V, Mashko, S V, Kotenko, V P, Veĭko, M I, Lebedeva, A L, Lapidus, A V, Mochul'skiĭ, I I, Shekhter, L S, Izotova, Iu P, Vinetskiĭ, V G, Debabov
Publikováno v:
Doklady Akademii nauk SSSR. 312(1)
Autor:
A L, Lapidus, A V, Mochul'skii, S M, Podkovyrov, M I, Lebedeva, A A, Antipin, L S, Izotova, O P, Zagnit'ko, G S, Komolova, A A, Klesov, V P, Veiko
Publikováno v:
Biomedical science. 1(6)
Recombinant human angiogenin has been synthesized in Escherichia coli with the aid of a human angiogenin gene (hAng) cloned by Neznanov et al (1990) from a human complementary DNA (cDNA) library. The gene has been expressed by use of a new type of ex
Autor:
Z. T. Abramov, Ermakova Lm, Valentin M. Stepanov, L.P. Belyanova, L. S. Izotova, A.Ya. Strongin, Ludmila A. Baratova, L.A. Lyublinskaya
Publikováno v:
Biochemical and Biophysical Research Communications. 77:298-305
Summary Intracellular serine protease was isolated in a pure state from sporulating Bacillus subtilis A-50. The enzyme has the molecular weight about 30.000 daltons and pI 4.3, is completely inhibited with phenylmethylsulfonyl fluoride and EDTA, and
Publikováno v:
Molecular and General Genetics MGG. 159:337-339
A Bacillus subtilis intracellular serine protease is homologous in sequence to an extracellular serine protease from the same strain, indicating the presence of two related structural genes in the Bacillus subtilis genome.
Publikováno v:
Biokhimiia (Moscow, Russia). 50(12)
Using radioimmunological methods based on the use of mono- and polyclonal antibodies raised against interferon alpha A, it was shown that polyclonal antibodies quantitatively reacted not only with this protein, but also with interferons alpha F and a
Publikováno v:
Biokhimiia (Moscow, Russia). 53(10)
Using stepwise ion-exchange and gel-permeation high performance liquid chromatography and SDS-PAAG gel electrophoresis, it was demonstrated that the non-reduced gene-engineered interferon alpha A is represented by multiple forms, namely, four monomer