Zobrazeno 1 - 2
of 2
pro vyhledávání: '"L. R. Fee"'
Publikováno v:
Protein Science. 4:2411-2423
The molar absorption coefficient, epsilon, of a protein is usually based on concentrations measured by dry weight, nitrogen, or amino acid analysis. The studies reported here suggest that the Edelhoch method is the best method for measuring epsilon f
Autor:
J. M. Scholtz, Gerald R. Grimsley, Beatrice M. P. Huyghues-Despointes, L. R. Fee, Roy W. Alston, Pace Cn, Richard L. Thurlkill, Kevin L. Shaw
Publikováno v:
Protein science : a publication of the Protein Society. 8(9)
It is difficult to increase protein stability by adding hydrogen bonds or burying nonpolar surface. The results described here show that reversing the charge on a side chain on the surface of a protein is a useful way of increasing stability. Ribonuc