Zobrazeno 1 - 10
of 48
pro vyhledávání: '"L. Kevin Lewis"'
Publikováno v:
Heliyon, Vol 9, Iss 1, Pp e13009- (2023)
Halloysite particles (HNTs) are naturally occurring aluminosilicate nanotubes of low toxicity that have shown great promise for drug and biomolecule delivery into human and animal cells. Kaolinite particles retain the same layered structure as HNT, b
Externí odkaz:
https://doaj.org/article/b4463c150bd1468384df534a61d8a40c
Publikováno v:
BioTechniques, Vol 48, Iss 4, Pp 297-302 (2010)
Absorbance spectroscopy is routinely used to monitor the concentrations of nucleic acids and proteins within solutions and to assess changes in their structure caused by interaction with chemicals or other biomolecules. Biological samples used for su
Externí odkaz:
https://doaj.org/article/a54101404ab445fba35d4955eb52e6a2
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2513
Transformation of DNA into cells of the budding yeast Saccharomyces cerevisiae and other industrially important yeasts is most commonly performed using chemical-based methods. Current protocols typically involve exposure of the cells to lithium ions
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::5a3e0e12b570470bd6bb3c97aa433d64
https://pubmed.ncbi.nlm.nih.gov/35781197
https://pubmed.ncbi.nlm.nih.gov/35781197
Publikováno v:
Methods in Molecular Biology ISBN: 9781071623985
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5909bbc1d2ff83964addd6edcc514d89
https://doi.org/10.1007/978-1-0716-2399-2_2
https://doi.org/10.1007/978-1-0716-2399-2_2
Autor:
Gary W. Beall, Shubha R.L. Malla, Benjamin R. Martin, L. Kevin Lewis, Tanner C. Gray, Corbin J. England, Samantha A. Oliveira
Publikováno v:
Anal Biochem
Precipitation of DNA is performed frequently in molecular biology laboratories for the purpose of purification and concentration of samples and also for transfer of DNA into cells. Metal ions are used to facilitate these processes, though their preci
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb74eef0ee9a29efd118bdcff4293cf3
https://pubmed.ncbi.nlm.nih.gov/33388294
https://pubmed.ncbi.nlm.nih.gov/33388294
Publikováno v:
Journal of Microbiological Methods. 133:46-51
Many plasmids used for gene cloning and heterologous protein expression in Escherichia coli cells are low copy number or single copy number plasmids. The extraction of these types of plasmids from small bacterial cell cultures produces low DNA yields
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d918f15bd25a7f7fb2e410e805e78b7d
https://doi.org/10.1016/j.mimet.2016.12.016
https://doi.org/10.1016/j.mimet.2016.12.016
Publikováno v:
Exp Cell Res
Telomerase-deficient cells of the budding yeast S. cerevisiae experience progressive telomere shortening and undergo senescence in a manner similar to that seen in cultured human fibroblasts. The cells exhibit a DNA damage checkpoint-like stress resp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f96defb0b8e3c13dcb106dd97bb1977f
https://pubmed.ncbi.nlm.nih.gov/31075257
https://pubmed.ncbi.nlm.nih.gov/31075257
Publikováno v:
Analytical Biochemistry. 492:69-75
High-quality chromosomal DNA is a requirement for many biochemical and molecular biological techniques. To isolate cellular DNA, standard protocols typically lyse cells and separate nucleic acids from other biological molecules using a combination of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c3412a2ccbd49a302c687ccafdc07fd6
https://doi.org/10.1016/j.ab.2015.09.017
https://doi.org/10.1016/j.ab.2015.09.017
Publikováno v:
The FASEB Journal. 34:1-1
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6cb4501208d61032c838d01846b837c6
https://doi.org/10.1096/fasebj.2020.34.s1.00460
https://doi.org/10.1096/fasebj.2020.34.s1.00460
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1855
Recent advances in agarose gel electrophoresis protocols established conditions for the high-resolution separation of DNA and RNA using higher voltages combined with short run times. We subsequently developed a protocol for using these conditions to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::6b19356141560e9f5763d43dfd74044e
https://pubmed.ncbi.nlm.nih.gov/30426432
https://pubmed.ncbi.nlm.nih.gov/30426432