Zobrazeno 1 - 10
of 37
pro vyhledávání: '"L. Kalachová"'
Autor:
Jan Kormanec, Marie Weiserova, M. Holub, Olga Kofroňová, Oldřich Benada, Jaroslav Weiser, Liem Nguyen, L. Kalachová
Publikováno v:
Folia Microbiologica. 50:393-400
We cloned EF-Tu from Streptomyces aureofaciens on a pET plasmid and overproduced it using the T7 RNA polymerase system in Escherichia coli. Streptomyces EF-Tu represented more than 40% of the total cell protein and was stored mostly in inclusion bodi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::969ab4d9e1f36b21a29d9d8bddcfe399
https://doi.org/10.1007/bf02931420
https://doi.org/10.1007/bf02931420
Autor:
Vorísek J, L. Kalachová
Publikováno v:
Folia Microbiologica. 48:767-770
The lead phosphate precipitation method showed the reaction product of acid phosphatase (which reflects the presence of the enzyme glycoprotein) in peripheral cytoplasmic vesicles in the ascomycetous fungusClaviceps purpurea. The product appeared to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::65d98de446db4ed985874f76fc84d7c0
https://doi.org/10.1007/bf02931511
https://doi.org/10.1007/bf02931511
Autor:
Zuzana Techniková, Silvia Bezoušková, Karolína Buriánková, Petr Halada, L. Kalachová, Jaroslav Weiser, Jiří Janeček, Denisa Petráčková, Kateřina Haladová
Publikováno v:
MicrobiologyOpen
We studied the impact of a sublethal concentration of erythromycin on the fitness and proteome of a continuously cultivated population of Escherichia coli. The development of resistance to erythromycin in the population was followed over time by the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::937ffe7f2319d6884fc18c73b9f5d6b5
https://pubmed.ncbi.nlm.nih.gov/23996919
https://pubmed.ncbi.nlm.nih.gov/23996919
Autor:
Olga Kofroňová, Silvia Bezoušková, Oldřich Benada, M. Holub, L. Kalachová, Jaroslav Weiser, Denisa Petráčková
Publikováno v:
Folia microbiologica. 55(3)
We present the results of analysis of membrane phosphoproteomes from individual morphological stages of Streptomyces coelicolor that reflect developmentally dependent heterogeneity and phosphorylation of intrinsic and externally added purified Strepo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c5a7daedba0f5c0b6f39b36b44043e5
https://pubmed.ncbi.nlm.nih.gov/20526830
https://pubmed.ncbi.nlm.nih.gov/20526830
Publikováno v:
Folia Microbiologica. 51
A reporter gene system, based on luciferase genes from Vibrio harvei, was constructed for measurement of translation nonsense suppression in Streptomyces. Using the site-directed mutagenesis the TCA codon in position 13 of the luxB gene was replaced
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8e6633bde5924a256c4d2f90682f9d23
https://doi.org/10.1007/bf02931452
https://doi.org/10.1007/bf02931452
Autor:
Olga Kofronova, L. Kalachová, Charles J. Thompson, Jana Novotná, Jaroslav Weiser, M. Holub, Liem Nguyen, Oldrich Benada
Publikováno v:
Applied and environmental microbiology. 71(6)
A two-phase cultivation system was developed which will enable studies of streptomycete differentiation by molecular biological and global techniques such as transcriptomics and proteomics. The system is based on a solid phase formed by glass beads c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a59a0a679b423e490c453f47d294ff8e
https://pubmed.ncbi.nlm.nih.gov/15932976
https://pubmed.ncbi.nlm.nih.gov/15932976
Publikováno v:
Biotechnology advances. 8(1)
Biosynthesis of chlortetracycline is localized differently under low- and high-production conditions (standard low-production strain and its high-production variant). The experimental evidence was based on the assay of anhydrotetracycline oxygenase i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1f52ac47bf82ed8837fe6f7f577e211b
https://pubmed.ncbi.nlm.nih.gov/14545907
https://pubmed.ncbi.nlm.nih.gov/14545907
Publikováno v:
Journal of Apicultural Research. 27:35-39
SummaryTwelve Bacillus larvae strains were searched for the presence of extrachromosomal DNA by agarose gel electrophoretic methods. Small plasmid molecules of a uniform size were detected in three of them. The molecular mass was determined at 5·72
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f4b6a382d41b853e164fb268952e6705
https://doi.org/10.1080/00218839.1988.11100779
https://doi.org/10.1080/00218839.1988.11100779
Publikováno v:
Journal of chromatography. 91
A method for the determination of the composition of the extracellular polysaccharides of some soil bacteria by gas chromatography was worked out. Monosaccharides originating from the hydrolysis of polysaccharides were separated as trimethylsilyl der
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::425ef27451a218bd4883b1a19eb1c1b3
https://pubmed.ncbi.nlm.nih.gov/4597580
https://pubmed.ncbi.nlm.nih.gov/4597580
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