Zobrazeno 1 - 10
of 19
pro vyhledávání: '"L. I. Rothblum"'
Publikováno v:
Journal of Biological Chemistry. 267:35-38
We have analyzed the expression, phosphorylation, and localization of the ribosomal DNA transcription factors UBF1 and UBF2 in Chinese hamster ovary cells in response to serum deprivation. In vivo labeling experiments demonstrate that UBF1 and UBF2 a
Publikováno v:
Nucleic Acids Research. 18:1677-1718
The spacer promoter of the rat rDNA repeat consists of two functional domains: a core (proximal) element that is sufficient for transcription in vitro, and an upstream (distal) promoter element that increases the efficiency of transcription. Two of t
Publikováno v:
Biochemistry and cell biology = Biochimie et biologie cellulaire. 76(4)
Transmission electron microscopy and single particle electron crystallography were employed to reconstruct high-quality projection images of a recombinant, acidic tail deficient form of rat upstream binding factor. The upstream binding factor was fou
Autor:
R D, Hannan, L I, Rothblum
Publikováno v:
Cardiovascular research. 30(4)
In summary, the increased capacity for protein synthesis that is a constant feature in all forms of cardiac hypertrophy is largely mediated by accelerated ribosome biogenesis. Experiments with neonatal cardiomyocytes in culture indicate that the acti
Publikováno v:
The Journal of biological chemistry. 268(19)
Two novel isoforms of the Ca2+/calmodulin-dependent protein kinase II delta subunit were detected in rat aorta. Identification of the subunits was based on two independent lines of evidence, i.e. detection by immunoblotting of differently sized delta
Publikováno v:
The Journal of biological chemistry. 267(1)
We have analyzed the expression, phosphorylation, and localization of the ribosomal DNA transcription factors UBF1 and UBF2 in Chinese hamster ovary cells in response to serum deprivation. In vivo labeling experiments demonstrate that UBF1 and UBF2 a
Autor:
W Q, Xie, L I, Rothblum
Publikováno v:
BioTechniques. 11(3)
A rapid and simple protocol for the isolation of RNA from transfected tissue culture cells is described. The protocol employs a guanidinium thiocyanate/phenol mixture to lyse cells directly from tissue culture plates and extract the total RNA. A tota
Autor:
H F Yang-Yen, L I Rothblum
Publikováno v:
Molecular and Cellular Biology. 8:3406-3414
A 16,000-dalton, high-mobility-group-like (HMG-like) DNA-binding protein, referred to as p16, has been purified to homogeneity from Novikoff hepatoma ascites cells. p16 binds specifically to a portion of the 5' flanking region of the rat rRNA gene (-
Publikováno v:
Molecular and Cellular Biology. 7:2388-2396
We identified and characterized an additional promoter within the nontranscribed spacer (NTS) of the rat ribosomal gene repeat that is capable of supporting initiation of transcription by RNA polymerase I in vitro. Within this promoter there is a seq
Publikováno v:
Nucleic Acids Research. 10:3667-3680
The DNA sequence of the intragenic region of the rat 45S ribosomal RNA precursor was determined. This sequence contains 2282 nucleotides and extends from the conserved EcoR I site near the 3' terminus of 18S rRNA to 69 nucleotides downstream of the 5