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Akademický článek

Characterization of Cme and Yme thermostable Cas12a orthologs

Autor: Ryan T. Fuchs, Jennifer L. Curcuru, Megumu Mabuchi, Audrey Noireterre, Peter R. Weigele, Zhiyi Sun, G. Brett Robb

Publikováno v: Communications Biology, Vol 5, Iss 1, Pp 1-16 (2022)

Two distinct Cas12a orthologs (Cme and Yme) from metagenomic sources are characterized. The enzymes are found to be thermotolerant and are compared to three mesophilic Cas12a enzymes (Lba, Fno and Asp).

Externí odkaz: https://doaj.org/article/2f051ab7df9a4bb69aca89664afee82f

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Akademický článek

A catalogue of biochemically diverse CRISPR-Cas9 orthologs

Publikováno v: Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)

A few bacterial Cas9 nucleases have been repurposed as genome editing tools. Here, the authors use bioinformatic and biochemical analyses to characterize 79 Cas9 proteins, revealing substantial functional diversity and thus expanding the available to

Externí odkaz: https://doaj.org/article/eff8b92a7b8248269794dd870fd5600c

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Development of a Cas12a-Based Genome Editing Tool for Moderate Thermophiles

Autor: Justin Albers, Megumu Mabuchi, Ryan T. Fuchs, Prarthana Mohanraju, Richard van Kranenburg, Jennifer L. Curcuru, Ioannis Mougiakos, G. Brett Robb, John van der Oost

Publikováno v: CRISPR Journal, 4(1), 82-91
CRISPR J
CRISPR Journal 4 (2021) 1

The ability of CRISPR-Cas12a nucleases to function reliably in a wide range of species has been key to their rapid adoption as genome engineering tools. However, so far, Cas12a nucleases have been limited for use in organisms with growth temperatures

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef649b486c311acffb3f3104cae4722f
https://doi.org/10.1089/crispr.2020.0086

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Characterization of Cme and Yme thermostable Cas12a orthologs

Autor: Ryan T, Fuchs, Jennifer L, Curcuru, Megumu, Mabuchi, Audrey, Noireterre, Peter R, Weigele, Zhiyi, Sun, G Brett, Robb

Publikováno v: Communications biology. 5(1)

CRISPR-Cas12a proteins are RNA-guided endonucleases that cleave invading DNA containing target sequences adjacent to protospacer adjacent motifs (PAM). Cas12a orthologs have been repurposed for genome editing in non-native organisms by reprogramming

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::d52de699594fead92ca25de292f5a59c
https://pubmed.ncbi.nlm.nih.gov/35388146

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Biochemically diverse CRISPR-Cas9 orthologs

CRISPR-Cas9 nucleases are abundant in microbes. To explore this largely uncharacterized diversity, we applied cell-free biochemical screens to rapidly assess the protospacer adjacent motif (PAM) and guide RNA (gRNA) requirements of novel Cas9 protein

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f6cdbeb4449b33160674b8f8cb468bb
https://doi.org/10.1101/2020.04.29.066654

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A catalogue of biochemically diverse CRISPR-Cas9 orthologs

Publikováno v: Nature communications, London : Nature Publishing Group, 2020, vol. 11, no. 1, art. no. 5512, p. [1-10]
Nature Communications
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)

Bacterial Cas9 nucleases from type II CRISPR-Cas antiviral defence systems have been repurposed as genome editing tools. Although these proteins are found in many microbes, only a handful of variants are used for these applications. Here, we use bioi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c339dbfa2e08fc6e4b32aa0ea45d7144
https://repository.vu.lt/VU:ELABAPDB74530963&prefLang=en_US

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Staphylococcus aureus Cas9 is a multiple-turnover enzyme

Autor: Megumu Mabuchi, Jennifer L. Curcuru, Paul Yourik, G. Brett Robb, Ryan T. Fuchs

Publikováno v: RNA. 25:35-44

Cas9 nuclease is the key effector of type II CRISPR adaptive immune systems found in bacteria. The nuclease can be programmed by a single guide RNA (sgRNA) to cleave DNA in a sequence-specific manner. This property has led to its widespread adoption

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::950f5f6766fc8cf2dcc8fb2817c475c1
https://doi.org/10.1261/rna.067355.118

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Cas12a trans-cleavage can be modulated in vitro and is active on ssDNA, dsDNA, and RNA

Autor: Paul Yourik, G. Brett Robb, Ryan T. Fuchs, Megumu Mabuchi, Jennifer L. Curcuru

CRISPR-Cas12a (Cpf1) are RNA-guided nuclease effectors of acquired immune response that act in their native organisms by cleaving targeted DNA sequences. Like CRISPR-Cas9 RNA-guided DNA targeting enzymes, Cas12a orthologs have been repurposed for gen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ef66678910f61923982f2006e19ab33
https://doi.org/10.1101/600890

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Pseudomonas aeruginosa exploits lipid A and muropeptides modification as a strategy to lower innate immunity during chronic lung infection in cystic fibrosis

Autor: C. Cigana, L. Curcuru’, M. R. Leone, T. Ieranò, N. I. Lorè, I. Bianconi, F. Cozzolino, M. L. Bernardini, A. Bragonzi, SILIPO, ALBA, LANZETTA, ROSA, MOLINARO, ANTONIO

Pseudomonas aeruginosa can establish life-long airways chronic infection in patients with cystic fibrosis (CF) with pathogenic variants distinguished from initially acquired strain. Here, we analysed chemical and biological activity of P. aeruginosa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od______3730::61a0987daa4eee6f1fb015b4db8ad391
http://hdl.handle.net/11588/426056

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Akademický článek

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