Výsledky vyhledávání - "L. B. Gul'ko"

[Design of the human tumor-associated VNTR(Muc1) antigen gene, fused with streptavidin, its expression in Escherichia coli. Study of properties of the hybrid protein]

Autor: L B, Gul'ko, O V, Pavlova, N A, D'iadkov, N A, Okorokova, K I, Ratmanova, N N, Logunova, R A, Bobreneva, V A, Makarov, V L, Iurin, V P, Veĭko, V G, Debabov

Publikováno v: Bioorganicheskaia khimiia. 26(6)

A gene of human tumor-associated antigen VNTR(MUC1) bound to streptavidin, an expression plasmid, and a highly effective hybrid protein-producing strain were constructed. It was shown that the streptavidin leader peptide ensures an effective secretio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::ab4d3ca40367b5209ce9b28eac605b16
https://pubmed.ncbi.nlm.nih.gov/10923190

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[Cloning transforming genes from human papillomavirus type 18]

Autor: M, Laasri, L B, Gul'ko, V P, Veĭko, F L, Kiselev

Publikováno v: Voprosy virusologii. 43(6)

Nucleotide sequences of type 18 human papilloma virus genes E6 and E7 inserted in human DNA cloned from cervical tumor are determined. The resultant sequences are compared to the prototype variant. Five point mutations not leading to replacement of a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::763253e0ba383a871d2de784e3f5b934
https://pubmed.ncbi.nlm.nih.gov/10488529

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[Cloning of streptavidin gene from Streptomyces avidinii and its expression in Escherichia coli. Secretion of streptavidin by E. coli cells]

Autor: V P, Veĭko, L B, Gul'ko, N A, Okorokova, N A, D'iakov, V G, Debabov

Publikováno v: Bioorganicheskaia khimiia. 25(3)

The streptavidin gene from Streptomyces avidinii was cloned, an expression plasmid constructed, and a highly effective strain producer of streptavidin created. It was shown that the leader peptide of streptavidin ensures the effective secretion of th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::bdf5a508f539d167ab016670a8c2df71
https://pubmed.ncbi.nlm.nih.gov/10382041

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[Protein engineering of uridine phosphorylase from Escherichia coli K-12. I. Cloning and expression of uridine phosphorylase genes from Klebsiella aerogenes and Salmonella typhimurium in E. coli]

Autor: V P, Veĭko, D V, Chebotaev, I V, Ovcharova, L B, Gul'ko

Publikováno v: Bioorganicheskaia khimiia. 24(5)

Genes of uridine phosphorylases (udp) from Klebsiella aerogenes and Salmonella typhimurium were cloned and expressed. Highly effective producer strains of the corresponding proteins were constructed. Enzymic properties of the UPases obtained were stu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::90d024637939779734dfffbd663912fd
https://pubmed.ncbi.nlm.nih.gov/9661793

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[Protein engineering of uridine phosphorylase from Escherichia coli K-12. Study of the role of cysteine residues in enzyme function]

Autor: V P, Veĭko, Z Z, Siprashvili, K I, Ratmanova, L B, Gul'ko, A A, Mironov

Publikováno v: Molekuliarnaia biologiia. 30(1)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::1ec087c237cbe56912df30c7c45f954b
https://pubmed.ncbi.nlm.nih.gov/8714134

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[Study of the role of histidine residues in the function of uridine phosphorylase from Escherichia coli K-12 by protein engineering]

Autor: V P, Veĭko, Z Z, Siprashvili, K I, Ratmanova, L B, Gul'ko

Publikováno v: Bioorganicheskaia khimiia. 21(11)

Using site-directed mutagenesis, mutant genes of the E.coli UDPase that coded proteins with point substitutions of histidine residues (i.e., H8N, H47N, H101N, H122N, H152N, H179N, and H240N) were constructed. Study of the enzymatic activity of mutant

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::6b46a891b62b65aaf23ffe6bffbd1fbe
https://pubmed.ncbi.nlm.nih.gov/8670308

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Molecular Genetic Investigation of Streptomyces Bambergiensis and Streptomyces Fradiae Strains, the Producers of Flavomycin and Tylosin

Autor: Sergey B. Zotchev, G. Y. Florova, Zhdanov Vg, Orekhov Av, A. R. Foors, L. B. Gul’ko, S. M. Rosenfeld

Publikováno v: Genetics and Product Formation in Streptomyces ISBN: 9781468459241

Streptomyces bambergiensis strains produce a phosphoglycolipid antibiotic complex flavomycin (moenomycin) [review Huber, 1978]. It is used in veterinary practice and is more active against Gram-positive bacteria. The antibiotics action mechanism is b

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::cf470dea85da6430c94d770d8ae8bf9b
https://doi.org/10.1007/978-1-4684-5922-7_17

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