Zobrazeno 1 - 10
of 22
pro vyhledávání: '"L. A. Chupova"'
Publikováno v:
Crystallography Reports. 60:682-684
Crystals of M. tuberculosis phosphopantetheine adenylyltransferase were grown in microgravity by the capillary counter-diffusion method through a gel layer. The X-ray diffraction data set suitable for the determination of the three-dimensional struct
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3494add5ae630a16091cfe68a4a31494
https://doi.org/10.1134/s106377451505017x
https://doi.org/10.1134/s106377451505017x
Publikováno v:
Acta Crystallographica Section D Biological Crystallography. 68:1660-1670
Structures of recombinant phosphopantetheine adenylyltransferase (PPAT) fromMycobacterium tuberculosis(PPATMt) in the apo form and in complex with the substrate ATP were determined at 1.62 and 1.70 Å resolution, respectively, using crystals grown in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f01b262bee423b3d9cb14e447e3a7a49
https://doi.org/10.1107/s0907444912040206
https://doi.org/10.1107/s0907444912040206
Publikováno v:
Protein & Peptide Letters. 19:479-484
An artificial gene consisting of seven copies of an oxytocinoyl-lysine encoding sequence arranged in a tandem was synthesized and inserted downstream of the SspDnaB intein gene in a pTWIN1 plasmid. The corresponding fusion protein Dnab-7oxy contained
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb60554e523871ee7f9592f7563616f6
https://doi.org/10.2174/092986612800190973
https://doi.org/10.2174/092986612800190973
Publikováno v:
Crystallography Reports. 57:96-104
Crystals of phosphopantetheine adenylyltransferase (PPAT) from Mycobacterium tuberculosis in the apo form and in complexes with coenzyme A (PPAT/CoA) and dephosphocoenzyme A (PPAT/dPCoA) were grown in microgravity by the capillary counter-diffusion m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::62325627627151dffc50fee702a5bd1e
https://doi.org/10.1134/s1063774512010142
https://doi.org/10.1134/s1063774512010142
Autor:
Stepanova Ev, V. G. Likhvantseva, K. A. Beirakhova, Roman S. Esipov, L. A. Chupova, Anatoly I. Miroshnikov
Publikováno v:
Russian Journal of Bioorganic Chemistry. 38:64-70
The pigment epithelium-derived factor (PEDF), a secreted 50 kDa glycoprotein, is one of the most potent endogenous inhibitors of angiogenesis. The fragment 44–77 of PEDF possesses the antiangiogenic properties of the full-sized protein and is a pot
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d9c52395731a8f32fd41c6c49cba48de
https://doi.org/10.1134/s1068162012010074
https://doi.org/10.1134/s1068162012010074
Autor:
E. A. Smirnova, Mikhail V. Kovalchuk, Vladimir I. Timofeev, Inna P. Kuranova, Yu. A. Abramchik, Roman S. Esipov, V. Kh. Akparov, L. A. Chupova
Publikováno v:
Crystallography Reports. 56:884-891
Crystals of phosphopantetheine adenylyltransferase from Mycobacterium tuberculosis, thymidine phosphorylase from Escherichia coli, carboxypeptidase T from Thermoactinomyces vulgaris and its mutant forms, and crystals of complexes of these proteins wi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::87f5329bf70accc5d82ed69da4ebe7f9
https://doi.org/10.1134/s1063774511050154
https://doi.org/10.1134/s1063774511050154
Publikováno v:
Crystallography Reports. 55:1050-1059
Recombinant phosphopantetheine adenylyltransferase from Mycobacterium tuberculosis (PPAT Mt), which was produced by a high-producing strain and purified to 99%, was used for the crystal growth of the complex of the enzyme with coenzyme A (CoA). Cryst
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::da72545f620fa0517c6498a30d3fd4eb
https://doi.org/10.1134/s1063774510060234
https://doi.org/10.1134/s1063774510060234
Autor:
Roman S. Esipov, Vasily N. Stepanenko, Maxim L. Filipenko, Uljana A. Boyarskikh, L. A. Chupova, Anatoly I. Miroshnikov
Publikováno v:
Protein Expression and Purification. 61:1-6
Chemical-enzymatic synthesis of human Epidermal Growth Factor (hEGF) cDNA has been performed, following by cloning into expression vector pTWIN1 (New England Biolabs). The resulting recombinant fusion protein expressed in Escherichia coli consisted o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78ef0087d65755c633ee8a56d3d5e060
https://doi.org/10.1016/j.pep.2008.05.009
https://doi.org/10.1016/j.pep.2008.05.009
Publikováno v:
Russian Journal of Bioorganic Chemistry. 33:227-232
An artificial gene encoding oxyntomodulin was obtained using chemical and enzymatic methods and cloned into Escherichia coli. A recombinant plasmid was constructed containing a hybrid oxyntomodulin gene and Ssp dnaB intein from Synechocystis sp. The
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e21b47f1b88781826eeaf0dcffc001d6
https://doi.org/10.1134/s1068162007020045
https://doi.org/10.1134/s1068162007020045
Publikováno v:
Protein & Peptide Letters. 13:343-347
Chemico-enzymatic synthesis and cloning in Esherichia coli of an artificial gene coding human glucagon was performed. Recombinant plasmid containing hybrid glucagons gene and intein Ssp dnaB from Synechocestis sp. was designed. Expression of the obta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6949624373682a947beb8b4c3ede5949
https://doi.org/10.2174/092986606775974320
https://doi.org/10.2174/092986606775974320