Zobrazeno 1 - 10
of 47
pro vyhledávání: '"L. A. BROMLEY"'
Publikováno v:
Clinical Chemistry. 53:1847-1854
Background: Unlabeled probe detection with a double-stranded DNA (dsDNA) binding dye is one method to detect and confirm target amplification after PCR. Unlabeled probes and amplicon melting have been used to detect small deletions and single-nucleot
Autor:
Shale Dames, L. Kathryn Bromley, Roger E. Smith, Marc G. Elgort, Karl V. Voelkerding, Maria Erali, Mark G. Herrmann
Publikováno v:
The Journal of Molecular Diagnostics. 8:16-21
A disposable 0.2-ml polymerase chain reaction (PCR) tube modified with an aluminum oxide membrane (AOM) has been developed for the extraction, amplification, and detection of nucleic acids. To assess the dynamic range of AOM tubes for real-time PCR,
Publikováno v:
Anaesthesia. 57:334-337
Summary The aim of this study was to determine the ethnic mix of those patients being pre-operatively screened for sickle cell disease in a London teaching hospital and to determine the rate of ␣carriage of sickle haemoglobin amongst those tested.
Publikováno v:
Anaesthesia. 55:247-250
We evaluated a single-use, disposable, carbon-dioxide-powered, needleless injector (J-Tip, National Medical Products Inc., CA, USA), which is claimed to deliver a virtually painless, subcutaneous injection. Seventy-two patients undergoing various typ
Autor:
Karl V. Voelkerding, Carl T. Wittwer, L. Kathryn Bromley, Mark G. Herrmann, Jacob D. Durtschi
Publikováno v:
Clinical Chemistry. 53:150-152
We have discovered that the DNA sample used in our recent paper (1) as a control heterozygote at the sickle cell locus of β-globin (17A→T, also known as HBB c.20A→T by HUGO nomenclature) contained an additional variant. Subsequent sequencing rev
Autor:
Karl V. Voelkerding, L. Kathryn Bromley, Carl T. Wittwer, Mark G. Herrmann, Jacob D. Durtschi
Publikováno v:
Clinical chemistry. 52(3)
Background: DNA melting analysis for genotyping and mutation scanning of PCR products by use of high-resolution instruments with special “saturation” dyes has recently been reported. The comparative performance of other instruments and dyes has n
Autor:
Roger E. Smith, Mark G. Herrmann, Jacob D. Durtschi, Maria Erali, Karl V. Voelkerding, Cathy A. Petti, L. Kathryn Bromley
Publikováno v:
Journal of medical microbiology. 54(Pt 9)
A membrane-filter-based, fluorescent Gram stain method for bacterial detection in cerebrospinal fluid samples was developed and evaluated as a rapid, sensitive alternative to standard Gram stain protocols. A recently developed, modified version of th
Publikováno v:
Anaesthesia. 58(11)
Publikováno v:
Anaesthesia. 57(4)
The aim of this study was to determine the ethnic mix of those patients being pre-operatively screened for sickle cell disease in a London teaching hospital and to determine the rate of carriage of sickle haemoglobin amongst those tested. We retrospe
Publikováno v:
Anaesthesia. 54(5)
The vasoactive properties of EMLA (eutectic mixture of local anaesthetic) and amethocaine were compared using Doppler colour ultrasound to measure vein diameter in 20 male volunteers. EMLA or amethocaine cream were applied over veins on the dorsum of