Výsledky vyhledávání - "L V, Memelova"

Photoaffinity Modification of Bacteriophage T7 DNA-Dependent RNA Polymerase with a Reaction Product Containing an Azido Derivative of UTP

Autor: V L Tunitskaia, Sergey N. Kochetkov, Skoblov IuS, L. V. Memelova

Publikováno v: Molecular Biology. 38:907-913

The possibility of using 5-[3-(E)-(4-azido-2,3,5,6-tetrafluorobenzamido)-propenyl-1]-UTP (N3-TFBP-UTP) as a photoaffinity modifier of T7 DNA-dependent RNA polymerase (RNAP) was demonstrated. The UTP derivative proved to act as an effective substrate

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::0548545722f9a9524cecf0df3bbf994c
https://doi.org/10.1023/b:mbil.0000049870.13689.2a

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Mutant T7 RNA polymerase is capable of catalyzing DNA primer extension reaction

Autor: D. A. Kostyuk, Sergey N. Kochetkov, S.V. Kochetkova, E. E. Rusakova, Vera L. Tunitskaya, L. V. Memelova

Publikováno v: FEBS Letters. 423:189-192

The mutant T7 RNA polymerase (T7 RNAP), containing two substitutions (Y639F, S641A) was earlier shown to utilize both rNTP and dNTP in a transcription-like reaction. In this report the ability of the enzyme to catalyze DNA primer extension reaction w

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0260599c5807f2f8684541b35b0491c4
https://doi.org/10.1016/s0014-5793(98)00058-1

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Deoxyribonucleotide-containing RNAs: a novel class of templates for HIV- 1 reverse transcriptase

Autor: L. V. Memelova, Ekaterina G. Kazantseva, Dmitry A. Kostyuk, Irina L. Shchaveleva, S. O. Gudima, Sergey N. Kochetkov, Olga I. Grishchenko

Publikováno v: Scopus-Elsevier

Deoxyribonucleotide-containing RNA-like polynucleotides (dcRNAs) were synthesized by mutant T7 RNA polymerase and their structures confirmed by sequencing. dcRNAs annealed with a 20mer oligodeoxyribonucleotide primer were tested as templates/primers

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77fd6cde45718005d49cd9466ad3f31c
https://doi.org/10.1093/nar/25.22.4614

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[Photoaffinity modification of bacteriophage T7 DNA-dependent RNA polymerase by the reaction product containing the azido derivative of UTP]

Autor: V L, Tunitskaia, L V, Memelova, Iu S, Skoblov, S N, Kochetkov

Publikováno v: Molekuliarnaia biologiia. 38(6)

The possibility of using the 5-[3-(E)-(4-azido-2,3,5,6-tetrafluorobenzamido)-propenyl-1]-UTP (N3-TFBP-UTP) as the affinity modificator of bacteriophage T7 DNA-dependent RNA polymerase was demonstrated. The UTP derivative used was rather efficient sub

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::335a629289af4318f2f11f1d55eaf100
https://pubmed.ncbi.nlm.nih.gov/15612594

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[Stability of human immunodeficiency virus to azidothymidine. II. Kinetic characteristics of 'AZT-resistant' mutant forms of reverse transcriptase]

Autor: D K, Pokholok, S O, Gudima, L V, Memelova, D S, Esipov, V O, Rechinskiĭ, S N, Kochetkov

Publikováno v: Biokhimiia (Moscow, Russia). 61(1)

Prolonged treatment of AIDS patients with azidothymidine results in the development of resistance to the drug which correlates with the appearance of point mutations in the reverse transcriptase (RT) coding region within the HIV-1 pol gene. Kinetic s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::ea9e43a6d72022f2e937208b56ae333f
https://pubmed.ncbi.nlm.nih.gov/8679772

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[Kinetic analysis of interaction of human immunodeficiency virus reverse transcriptase with alkaloids]

Autor: S O, Gudima, L V, Memelova, V B, Borodulin, D K, Pokholok, B M, Mednikov, O N, Tolkachev, S N, Kochetkov

Publikováno v: Molekuliarnaia biologiia. 28(6)

The interactions of HIV-I reverse transcriptase with some alkaloids were studied. Among nine compounds tested three--berberine, palmatine and sanguiritrine--inhibited RT. The dependence of the inhibition on the type of template-primer was also demons

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::6cbba5f5b58217ec4d88dad72cdcdb4c
https://pubmed.ncbi.nlm.nih.gov/7533889

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[Studies of the resistance of the human immunodeficiency virus to azidothymidine. I. Kinetic studies of the interaction between reverse transcriptase and a series of its mutant forms with substrates and azidothymidine-5'-trisphosphate]

Autor: D K, Pokholok, S O, Gudima, D S, Esipov, V N, Dobrynin, L V, Memelova, V O, Rechinskiĭ, S N, Kochetkov

Publikováno v: Biokhimiia (Moscow, Russia). 59(5)

Prolonged therapy of AIDS patients with azidothymidine results in the development of resistance to the drug. This phenomenon is accompanied by the appearance of point mutations in the pol gene coding for reverse transcriptase (RT). Kinetic studies of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::c0bd7ea562d795240fde0546af501241
https://pubmed.ncbi.nlm.nih.gov/7519060

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[Functional studies of mutant forms of bacteriophage T7 RNA polymerases containing point substitutions in the motif at the active site of the enzyme]

Autor: V L, Tunitskaia, S M, Dragan, D A, Kostiuk, D L, Liakhov, L V, Memelova, V O, Rechinskiĭ, S N, Kochetkov

Publikováno v: Biokhimiia (Moscow, Russia). 59(4)

Monomeric DNA and RNA polymerases contain a number of conserved motifs. By means of random and oligonucleotide-directed mutagenesis the point mutants of bacteriophage T7 RNA polymerase containing the amino acid substitutions in motif B which is the p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::15306cb271960afc679371e669d40a22
https://pubmed.ncbi.nlm.nih.gov/8018771

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