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Biochemical properties of a highly purified v-rasH p21 protein overproduced in Escherichia coli and inhibition of its activities by a monoclonal antibody

Autor: Thomas Y. Shih, S Hattori, K Halliday, L S Ulsh

Publikováno v: Molecular and Cellular Biology. 5:1449-1455

The v-rasH oncogene of Harvey murine sarcoma virus encodes a 21,000-dalton p21 protein which has been expressed at a high level as a fusion protein in Escherichia coli. We have purified the p21 to over 90% in purity without the use of any detergent o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33402c9980e4a20e83ddf4eaa0a14742
https://doi.org/10.1128/mcb.5.6.1449

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Metabolic turnover of human c-rasH p21 protein of EJ bladder carcinoma and its normal cellular and viral homologs

Autor: T Y Shih, L S Ulsh

Publikováno v: Molecular and Cellular Biology. 4:1647-1652

The EJ bladder carcinoma oncogene is activated by a point mutation in the c-rasH proto-oncogene at the 12th amino acid codon. In an attempt to understand the mechanism of oncogenic activation, a comparative study was undertaken to examine the metabol

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b6dd2e777bb374cda88848720e9c03d
https://doi.org/10.1128/mcb.4.8.1647

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Posttranslational processing of p21 ras proteins involves palmitylation of the C-terminal tetrapeptide containing cysteine-186

Autor: Z Q Chen, L S Ulsh, G DuBois, T Y Shih

The p21 proteins of ras oncogenes are synthesized as precursors in the cytosol. After processing, which involves acylation, the products are associated with the plasma membrane in eucaryotic cells. The p21 overproduced in Escherichia coli, however, i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81396d060b51f815c8bec978dba2e610
https://europepmc.org/articles/PMC252618/

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Structure and function of p21 ras proteins

Autor: T Y, Shih, S, Hattori, D J, Clanton, L S, Ulsh, Z Q, Chen, J A, Lautenberger, T S, Papas

Publikováno v: Gene amplification and analysis. 4

Cancer is a malfunction of cellular growth control. The discovery of oncogenes, first in transforming retroviruses, and later in human and animal tumors, may have uncovered the key to understanding one of the most elusive subjects of basic cell biolo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::07eba33728f9e20f68469c7e81d04e27
https://pubmed.ncbi.nlm.nih.gov/3333361

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Novel phosphorylation of c-ras p21 by protein kinases

Autor: P, Saikumar, L S, Ulsh, D J, Clanton, K P, Huang, T Y, Shih

Publikováno v: Oncogene research. 3(3)

Novel p21 phosphorylation was found in cells expressing high levels of this product of c-H- and c-K-ras genes. Phorbol 12,13-dibutyrate, a protein kinase C (PKC) activator, and permeable c-AMP derivatives, which activate protein kinase A (PKA), stimu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::b78c59f4b1fd1e694dc7c4a1488eaf5f
https://pubmed.ncbi.nlm.nih.gov/2849744

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