Zobrazeno 1 - 7
of 7
pro vyhledávání: '"L R, Micklem"'
Publikováno v:
Thrombosis and haemostasis. 57(1)
Murine monoclonal antibodies to human von Willebrand factor (vWf) were immobilised on Sephacryl S-1000. Various solutes were screened for their ability to elute 125I-vWf from the immobilised antibodies. The most effective solutions were then tested t
Publikováno v:
Thrombosis and haemostasis. 53(1)
Seven mouse monoclonal antibodies have been produced against human melanoma tissue plasminogen activator (t-PA). They were specifically bound to 125I t-PA but not 125I urokinase (u-PA) and inhibited t-PA, but not u-PA, activity in plasminogen-rich 12
Autor:
C M, Sturgeon, L R, Micklem, A J, Hussey, J, Seth, A, Scott, A W, Ritchie, G D, Chisholm, K, James
Publikováno v:
Developments in biological standardization. 57
Hybridoma cells secreting anti-PAP were produced by fusion of NS-1 myeloma cells with spleen cells from immunized Balb/c mice. Three of 32 hybrids secreted antibodies. Dilution cloning of the two hybrids producing the highest antibody titres showed t
Publikováno v:
Thrombosis and haemostasis. 54(2)
Five monoclonal antibodies to human von Willebrand factor were selected for characterization from 18 produced in murine hybridomas. All showed a high and specific affinity for human von Willebrand factor (vWf) but exhibited little if any cross-reacti
Publikováno v:
Thrombosis and haemostasis. 55(1)
A panel of 10 murine monoclonal antibodies to procoagulant FVIII has been developed from the fusion of a single spleen. Balb/c mice were injected with a purified preparation of FVIII: Ag, and antibody production in sera and hybrid culture supernatant
Publikováno v:
Developments in biological standardization. 57
The production of relatively large amounts of pure monoclonal antibodies (MAb) has facilitated the development of MAb-based immunometric assays for a variety of clinically important analytes. 'Two-site' heterogeneous assays are now available which in
Publikováno v:
Journal of immunological methods. 118(1)
The production of nine monoclonal antibodies to human atrial natriuretic factor (ANF 1-28) is described. All possible combinations of two antibodies failed to reveal any which could simultaneously bind ANF. Studies with ANF analogues and the antibodi