Zobrazeno 1 - 10
of 15
pro vyhledávání: '"L M, Firsov"'
Autor:
T. N. Kozhina, Marina Surzhik, S. V. Churkina, L. M. Firsov, A. E. Shmidt, Michael Petukhov, D. L. Firsov, Alexey V. Shvetsov
Publikováno v:
Applied Biochemistry and Microbiology. 46:206-211
Conformational flexibility of α-helices in glucoamylase of the fungus Aspergillus awamori was studied by molecular dynamics methods. Several amino acid substitutions (G127A, P128A, I136L, G137A, and G139A) optimizing intrinsic interactions in one of
Publikováno v:
Biochemistry (Moscow). 68:1012-1019
Here we describe cloning, expression, and purification of the enzyme trehalose-6-phosphate hydrolase from thermoresistant strain Bacillus sp. GP16. Principal biochemical properties of the enzyme at different pH and temperature values were determined.
Publikováno v:
Biochemistry. Biokhimiia. 68(9)
Here we describe cloning, expression, and purification of the enzyme trehalose-6-phosphate hydrolase from thermoresistant strain Bacillus sp. GP16. Principal biochemical properties of the enzyme at different pH and temperature values were determined.
Publikováno v:
Biochemistry. Biokhimiia. 66(3)
This review highlights the main properties of mammalian, plant, and microbial alpha-glucosidases. Special attention is given to the classification of these enzymes, possible catalytic mechanisms, their tertiary structure, and the structure of major i
Publikováno v:
The Journal of biological chemistry. 269(22)
The three-dimensional structure of the pseudotetrasaccharide acarbose complexed with glucoamylase II(471) from Aspergillus awamori var. X100 has been determined to 2.4-A resolution. The model includes residues corresponding to 1-471 of glucoamylase I
Publikováno v:
Biokhimiia (Moscow, Russia). 58(6)
The possibility of cleavage of the alpha-bond between mannose (or galactose) and serine (or threonine) in the presence of alpha-mannosidase and alpha-galactosidase has been studied. Using model compounds simulating the O-glycosyl bond in glycoprotein
Publikováno v:
Biochemistry and molecular biology international. 30(1)
Upon secretion, the major form of glucoamylase from Asp. awamori adsorbs on fungal cell walls. Its substrate binding site showing affinity for alpha-1-4 and alpha-1-6 wall glucans is responsible for adsorption. The minor form in which this site is ab
Publikováno v:
The Journal of biological chemistry. 267(27)
The crystal structure of a catalytically active fragment of glucoamylase-I from Aspergillus awamori var. X100 has been determined to a resolution of 2.2 A. Twelve of its 13 alpha-helices are arranged into an "alpha/alpha-barrel." An inner core of six
Publikováno v:
Biokhimiia (Moscow, Russia). 41(7)
The points of bonds ruptures in the course of phosphoglucomutase reaction were studied by two methods: incorporation of 18O and kinetic isotope effect with the use of double label (14C and 3H). The method of 18O determination by means of activation a
Publikováno v:
Molecular biology. 8(4)
We have measured the alpha-deuterium and alpha-tritium secondary kinetic isotope effects on the synthesis and phosphorolysis of glycogen catalyzed by glycogen phosphorylase B (EC 2.4.1.1). The isotope-labeled substrates glucose-1T-1-phosphate and 1C1