Zobrazeno 1 - 10
of 16
pro vyhledávání: '"L Iu, Brovko"'
Publikováno v:
Prikladnaia biokhimiia i mikrobiologiia. 35(3)
A rapid (30-35 min) bioluminescence assay of total bacterial contamination (TBC) of raw milk was optimized. This method includes incubation of milk samples in the presence of Neonol-10 and medical purity grade pancreatin with further removal of nonba
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::da4251df7cb91673fe8ccf4a855942ba
https://pubmed.ncbi.nlm.nih.gov/10496009
https://pubmed.ncbi.nlm.nih.gov/10496009
Publikováno v:
Prikladnaia biokhimiia i mikrobiologiia. 33(4)
A bioluminescence assay for rapid (5-7 h) determination of susceptibility to antibiotics was applied to samples of septic blood and optimized. The method comprises hemolysis of blood, reduction of osmolarity by adding concentrated nutritive media, an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::7c91b66fd8e183fa4507a69dfebec08f
https://pubmed.ncbi.nlm.nih.gov/9380653
https://pubmed.ncbi.nlm.nih.gov/9380653
Publikováno v:
Prikladnaia biokhimiia i mikrobiologiia. 33(3)
The efficiency of dimethyl sulfoxide (DMSO), trichloroacetic acid (TCA), and cetyltrimethylammonium bromide (CTAB) as extractants of intracellular ATP from various microorganisms was compared in bioluminescent measurements of microbial cell concentra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::cac829527af0aa62c308ec73d609da4d
https://pubmed.ncbi.nlm.nih.gov/9297187
https://pubmed.ncbi.nlm.nih.gov/9297187
Publikováno v:
Biokhimiia (Moscow, Russia). 58(9)
Recent data from gene engineering, kinetic and fluorescent studies concerning the structure and functions of firefly luciferase are reviewed. Some new trends in the development of bioluminescent methods of control over bacterial contaminations, dynam
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::15c3fd7252ae7b41f1d6da3795bf1000
https://pubmed.ncbi.nlm.nih.gov/8218559
https://pubmed.ncbi.nlm.nih.gov/8218559
Publikováno v:
Biokhimiia (Moscow, Russia). 58(3)
Evidence is presented of co-immobilized bioluminescent reagents which include firefly luciferase, pyruvate kinase and adenylate kinase used for an adenylate assay in bacterial cells. The changes in the ATP, ADP and AMP content induced by irradiation
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::0e582cc47449d1c3adca5168a7b80c04
https://pubmed.ncbi.nlm.nih.gov/8485225
https://pubmed.ncbi.nlm.nih.gov/8485225
Autor:
I M, Parkhomenko, G V, Perishvili, V B, Turovetskiĭ, Iu B, Kudriashov, A B, Rubin, L Iu, Brovko
Publikováno v:
Radiobiologiia. 33(1)
X irradiation of Chinese fibroblasts with doses of 0.05-0.15 Gy was shown to cause intracellular pH (pHi) changes: its diminishing during the first 40-60 min by 0.16-0.18 pH units, then the return to the control level 120 min after irradiation and, f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::e3aaa2ca19dcb43e73fb1f3a339cfaa1
https://pubmed.ncbi.nlm.nih.gov/8469730
https://pubmed.ncbi.nlm.nih.gov/8469730
Autor:
S Iu, Kondrashov, L I, Shirina, V V, Krzhechkovskaia, L Iu, Brovko, V B, Turovetskiĭ, V K, Mazo
Publikováno v:
Voprosy pitaniia. (5-6)
Histamine secretion and ATP levels were measured in mast cells from the abdominal and pleural cavities of Wistar rat males (250-300 g) which had been starving for 2-4 days. Food deprivation had no effect on histamine levels and secretion in exposure
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::a369c75ec87c69a70d2d4ae042de9b09
https://pubmed.ncbi.nlm.nih.gov/1284259
https://pubmed.ncbi.nlm.nih.gov/1284259
Publikováno v:
Laboratornoe delo. (10)
A bioluminescent rapid method (a three-hour test) has been developed to assess the susceptibility of microflora to antibiotics. It is based on comparison of intracellular ATP content in a sample (pus) after 3 h incubation at 37 degrees C in liquid cu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::56d2a9110262938c358528c5108713cb
https://pubmed.ncbi.nlm.nih.gov/1704465
https://pubmed.ncbi.nlm.nih.gov/1704465
Publikováno v:
Antibiotiki i meditsinskaia biotekhnologiia = Antibiotics and medical biotechnology. 31(2)
The possible applications of the immobilized bioluminescent systems of bacteria and fireflies in microassay are shown. The immobilization resulted in 10-100-fold stabilization of luciferases permitting their multiple use in flow column reactors. Reag
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::f571d62f7df524f0580a08dc31f2fc53
https://pubmed.ncbi.nlm.nih.gov/3963794
https://pubmed.ncbi.nlm.nih.gov/3963794
Publikováno v:
Biokhimiia (Moscow, Russia). 45(9)
The luciferase from the fireflies luciola mingrelica was immobilized on different CNBr-activated polysaccharide carriers, e. g. Sepharose, Ultrodex, cellophane, Ultrogel. The catalytic activity and the inactivation rate constants of the soluble and i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::59bf3ffe8dcc5b3ed3b02d3a91568c94
https://pubmed.ncbi.nlm.nih.gov/7248359
https://pubmed.ncbi.nlm.nih.gov/7248359