Zobrazeno 1 - 10
of 13
pro vyhledávání: '"L I, Binder"'
Publikováno v:
Journal of Histochemistry & Cytochemistry. 41:609-616
For accurate and quantitative immunohistochemical localization of antigens it is crucial to know the solubility of tissue proteins and their degree of loss during processing. In this study we focused on the solubility of several cytoskeletal proteins
Publikováno v:
Journal of Biological Chemistry. 267:564-569
Tau (tau) is a major constituent of paired helical filaments (PHF) found in Alzheimer's disease. The current study examines the possibility that the distinct properties of PHF-associated tau proteins (tau PHF) result from post-translational modificat
Autor:
N, Ghoshal, F, García-Sierra, Y, Fu, L A, Beckett, E J, Mufson, J, Kuret, R W, Berry, L I, Binder
Publikováno v:
Journal of neurochemistry. 77(5)
We have characterized a novel monoclonal antibody, Tau-66, raised against recombinant human tau. Immunohistochemistry using Tau-66 reveals a somatic-neuronal stain in the superior temporal gyrus (STG) that is more intense in Alzheimer's disease (AD)
Publikováno v:
Cell motility and the cytoskeleton. 38(1)
The Tau-1 monoclonal antibody was localized to the nucleolus of interphase cells and the nucleolar organizing regions (NORs) of acrocentric chromosomes in cultured human cells. Putative nucleolar and NOR tau was found in CG neuroblastoma cells which
Publikováno v:
Journal of neurochemistry. 63(6)
Microtubule-associated protein 2 (MAP-2) is an abundant neuronal cytoskeletal protein that binds to tubulin and stabilizes microtubules. Using fusion protein constructs we have defined the epitopes of 10 monoclonal antibodies (mAbs) to discrete regio
Publikováno v:
The Journal of biological chemistry. 267(1)
Tau (tau) is a major constituent of paired helical filaments (PHF) found in Alzheimer's disease. The current study examines the possibility that the distinct properties of PHF-associated tau proteins (tau PHF) result from post-translational modificat
Publikováno v:
Journal of neuroscience research. 25(3)
Alz 50, a monoclonal antibody raised against Alzheimer brain homogenate, reacts with neurofibrillary tangles, microtubule-associated proteins tau, and Alzheimer brain proteins of molecular weight 70-60 kDa (A68). To study the relationship between A68
Publikováno v:
Journal of Biological Chemistry. 263:7948-7953
Microtubule-associated protein tau was characterized in 5 Alzheimer and 5 control brains using two monoclonal antibodies, Alz 50 and Tau-1. Quantitative analysis of immunoblots with the antibodies showed that both homogenate and supernatant fractions
Autor:
J L Rosenbaum, L I Binder
Publikováno v:
The Journal of Cell Biology
Flagellar outer doublet microtubules were solubilized by use of sonication, and the tubulin was reassembled in vitro into single microtubules containing 14 and 15 protofilaments. The tubulin assembly was dependent on both the KCl and tubulin concentr
Publikováno v:
Science (New York, N.Y.). 185(4148)
Basal bodies isolated from Chlamydomonas reinhardi will serve as initiation centers for the assembly of chick brain microtubule protein subunits (tubulin) into microtubules. The rate of microtubule assembly is tubulin-concentration dependent; this as