Zobrazeno 1 - 8
of 8
pro vyhledávání: '"L G, Bachas"'
Publikováno v:
Biotechnology and bioengineering. 65(4)
Horseradish peroxidase (HRP) was biotinylated with biotinamidocaproate N-hydroxysuccinimide ester (BcapNHS) in a controlled manner to obtain biotinylated horseradish peroxidase (Bcap-HRP) with two biotin moieties per enzyme molecule. Avidin-mediated
Publikováno v:
Biotechnology and bioengineering. 60(5)
Kinetic comparisons have been made between a randomly immobilized and a site-specifically immobilized subtilisin BPN' on microfiltration membranes of varying hydrophilicities in both aqueous and organic media. Site-directed mutagenesis was employed t
Autor:
N G, Hentz, L G, Bachas
Publikováno v:
Methods in enzymology. 279
Publikováno v:
Analytical chemistry. 68(8)
Heparin (or hydrolyzed heparin) was covalently attached on the surface of derivatized cellulose triacetate membranes, which were subsequently impregnated with the potassium-selective ionophore valinomycin. The resulting ion-selective electrodes prese
Autor:
M S, Barbarakis, L G, Bachas
Publikováno v:
Clinical chemistry. 37(1)
The degree of substitution of protein-ligand conjugates can be determined from the change of the isoelectric point (pI) of the protein when ligand molecules are attached to its surface. Specifically, the pI values of conjugates with known degrees of
Publikováno v:
Clinical chemistry. 36(7)
We describe a simple method for estimating the association constant between an antibody and an antigen, based on a theoretical treatment of experimental dose-response data. We used this method to calculate the association constants of an anti-progest
Publikováno v:
Progress in clinical and biological research. 292