Výsledky vyhledávání - "L E, Petrovskaia"

[Fluorescent fusion proteins with 10th human fibronectin domain]

Autor: L E, Petrovskaia, S Sh, Gapizov, L N, Shingarova, E A, Kriukova, E F, Boldyreva, S A, Iakimov, E V, Svirshchevskaia, E P, Lukashev, D A, Dolgikh, M P, Kirpichnikov

Publikováno v: Bioorganicheskaia khimiia. 40(4)

In the current paper we describe a new type of hybrid molecules including red fluorescent protein mCherry and 10th type III human fibronectin domain (10Fn3) - one of the alternative scaffold proteins which can be used for the construction of antibody

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::4ceb3de8ac8dbe7a45e66dd167386ced
https://pubmed.ncbi.nlm.nih.gov/25898750

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[Production and properties of human tumor necrosis factor peptide fragments]

Autor: L N, Shingarova, L E, Petrovskaia, A N, Nekrasov, E A, Kriukova, E F, Boldyreva, S A, Iakimov, S V, Gur'ianova, D A, Dolgikh, M P, Kirpichnikov

Publikováno v: Bioorganicheskaia khimiia. 36(3)

The tumor necrosis factor (TNF) is a proinflammatory cytokine that plays a pivotal role in the regulation of the human immune system. Studies of the TNF functional topography are a challenging task in bioengineering. We have produced genes encoding t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::c633d2ac9d84840f53a245896c007d20
https://pubmed.ncbi.nlm.nih.gov/20644587

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[Rapid and efficient extraction of soluble proteins from gram-negative microorganisms without disruption of cell walls]

Autor: V. N. Danilevich, L E Petrovskaia, E. V. Grishin

Publikováno v: Bioorganicheskaia khimiia. 32(6)

The ability of buffer solutions containing low concentrations of nonionic detergents (Triton X-100, Tween 20, Brij 58, and Lubrol PX) and the anionic detergent sodium deoxycholate, as well as mixtures of these detergents with chaeotropes (urea and gu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::36c6e85f3b5cae1ca51a2660f8538e64
https://pubmed.ncbi.nlm.nih.gov/17180908

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[Deletion mutants of human granulocyte-macrophage colony-stimulating factor]

Autor: L E, Petrovskaia, E A, Kriukova, A L, Kaiushin, A V, Rodina, E Iu, Moskaleva, V G, Korobko

Publikováno v: Bioorganicheskaia khimiia. 28(5)

To study the structure-function relationship of the human granulocyte-macrophage colony-stimulating factor (GM-CSF), genes were constructed that encode its three deletion mutants: D1, a mutant with the deletion of six amino acid residues (37-42) some

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::fc1dfcc26379e0a0ad5b40c74c9f5501
https://pubmed.ncbi.nlm.nih.gov/12408028

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[Chaperone Caf1M stabilizes hybrid proteins containing sequences of F1 antigen subunit from Yersinia pestis]

Autor: L E, Petrovskaia, E A, Kriukova, A L, Kaiushin, V G, Korobko

Publikováno v: Bioorganicheskaia khimiia. 27(4)

The Yersinia pestis (causative agent of plague) capsule antigen is a homopolymer of Caf1 protein. Export of the subunits is mediated by the periplasmic chaperone Caf1M. To study the mechanism of Caf1M activity, two hybrid genes including coding seque

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::ba7affe5f2e69f5d068a5910bd189f58
https://pubmed.ncbi.nlm.nih.gov/11558261

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[Immunoenzyme determination of human granulocyte-macrophage colony-stimulating factor using monoclonal antibodies]

Autor: O E, Lakhtina, L E, Petrovskaia, V G, Korobko, V A, Nemeianov

Publikováno v: Bioorganicheskaia khimiia. 25(9)

A set of seven hybridomas producing monoclonal antibodies (MAbs) to the human recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF) was obtained. The properties of the monoclonal antibodies were characterized, and pairs of MAbs specif

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::b3e860fdd44fd275933b395c8ebeee29
https://pubmed.ncbi.nlm.nih.gov/10624559

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[Effect of the topography of the signal peptidase site on the effectiveness of secretion of recombinant human granulocyte-macrophage colony-stimulating factor into Escherichia coli periplasm]

Autor: L E, Petrovskaia, E A, Kriukov, S A, Iakimov, A N, Vul'fson, R A, Alibaeva, L N, Shingarova, A A, Guzaev, V M, Abramov, V G, Korobko

Publikováno v: Bioorganicheskaia khimiia. 21(12)

Synthesis of an artificial gene encoding the signal peptide of the Yersinia pestis capsule antigen (Caf1) was accomplished. A set of plasmids coding for hybrid proteins in which a modified sequence of the Caf1 signal peptide is connected to the amino

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::581f70d14d5795078cbfb0562a6a3840
https://pubmed.ncbi.nlm.nih.gov/8602887

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[Design of recombinant Escherichia coli strains, determining the secretory expression of artificial human granulocyte-macrophage colony-stimulating factor genes]

Autor: L E, Petrovskaia, A V, Ruzin, L N, Shingarova, V G, Korobko

Publikováno v: Bioorganicheskaia khimiia. 21(11)

A number of recombinant plasmids for expression of artificial genes encoding human granulocyte-macrophage colony stimulating factor (GM-CSF) were constructed. A hybrid gene was obtained that contains a sequence encoding the leader peptide and a tande

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::ec16c727c7ea07fa7676536747530d70
https://pubmed.ncbi.nlm.nih.gov/8670310

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