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Molecular cloning and expression of aPenicillium citrinumallergen with sequence homology and antigenic crossreactivity to a hsp 70 human heat shock protein

Autor: L.-C. Au, J.-J. Tsai, S.-F. Liaw, S.-H. Han, H.-D. Shen, W.-L. Lin

Publikováno v: Clinical & Experimental Allergy. 27:682-690

Summary BackgroundPenicillium citrinum has been identified as the most prevalent airborne Penicillium species in the Taipei area. However, detailed studies on allergens of this ubiquitous Penicillium species are still lacking. Objective For the chara

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::2f3085f1892cf7ad7498252cb13dfafb
https://doi.org/10.1111/j.1365-2222.1997.tb01197.x

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L-amino-acid oxidase from the Malayan pit viper Calloselasma rhodostoma. Comparative sequence analysis and characterization of active and inactive forms of the enzyme

Autor: P, Macheroux, O, Seth, C, Bollschweiler, M, Schwarz, M, Kurfürst, L C, Au, S, Ghisla

Publikováno v: European journal of biochemistry. 268(6)

Here we report the cDNA-deduced amino-acid sequence of L-amino-acid oxidase (LAAO) from the Malayan pit viper Calloselasma rhodostoma, which shows 83% identity to LAAOs from the Eastern and Western diamondback rattlesnake (Crotalus adamanteus and Cro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::241e02b2502b9306a576876687302739
https://pubmed.ncbi.nlm.nih.gov/11248687

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Suppression of vincristine-mediated cytotoxic activity by mitoxantrone in human cell lines

Autor: C K, Ho, L C, Au, S Y, Wang

Publikováno v: Anticancer research. 16(5A)

In the present study, we used different cell lines to determine the anticellular effect of a combination of mitoxantrone (MXT) and vincristine (VCR). In all the cell lines tested, most cells (approximately 90%) in cultures with VCR (0.01-1 microM) al

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::51336df7ca50568340612d5a2e46aa23
https://pubmed.ncbi.nlm.nih.gov/8917364

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Molecular analysis of cellular loci disrupted by papillomavirus 16 integration in cervical cancer: frequent viral integration in topologically destabilized and transcriptionally active chromosomal regions

Autor: K B, Choo, C M, Chen, C P, Han, W T, Cheng, L C, Au

Publikováno v: Journal of medical virology. 49(1)

To discern the structural features of cellular loci that are disrupted by type 16 human papillomavirus (HPV-16) integration in cervical cancer, a polymerase chain reaction (PCR)-based strategy was employed for direct amplification and sequence analys

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::7de611c78dce1b3b9cbc62193030f126
https://pubmed.ncbi.nlm.nih.gov/8732866

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Single-step hybridization screening for recombinant DNA clones with correct insert orientation and intact junction using a junctional oligonucleotide probe

Autor: L C, Au, T Y, Lo, H H, Wang, K B, Choo

Publikováno v: Proceedings of the National Science Council, Republic of China. Part B, Life sciences. 20(2)

Cloning of DNA fragments with blunt ends or identical protruding ends results in colonies with the insertion sequence existing in two possible orientations. The orientation and the junctional sequence of the positive clones need to be established by

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::0854ec6b1a10ea98065e30d552eacb9a
https://pubmed.ncbi.nlm.nih.gov/8931341

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A simple and rapid method for purification of oligodeoxyribonucleoside methylphosphonates

Autor: S B, Lin, G W, Chang, G W, Teh, K I, Lin, L C, Au

Publikováno v: BioTechniques. 14(5)

An alternative dimethoxytrityl-on (dmt-on) method is described to purify hydrophobic oligodeoxyribonucleoside methyl-phosphonates (OM) with a phosphodiester linkage at the 5' end, instead of the conventional dmt-off method using a DEAE ion-exchange c

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https://pubmed.ncbi.nlm.nih.gov/8512705

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Rapid verification of lambda-cDNA clones using mixed-base oligonucleotide as screening probe and sequencing primer

Autor: L C, Au, Y B, Huang, G W, Teh, T F, Huang, K B, Choo

Publikováno v: Proceedings of the National Science Council, Republic of China. Part B, Life sciences. 14(4)

A rapid procedure has been developed for the isolation and verification of cDNA clones isolated from a cDNA library based on lambda vectors. Using information of the partial amino acid sequence of a protein, synthetic mixed-base oligonucleotides are

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::7949597f72bc32832266e51a4d2892f5
https://pubmed.ncbi.nlm.nih.gov/2151655

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Gene probe coated piezoelectric biosensors for biochemical analysis

Autor: T Z, Wu, H H, Wang, L C, Au

Publikováno v: Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology. 23(2)

A new method has been developed to monitor DNA hybridization by using piezoelectric (PZ) crystal biosensor system. AT-cut crystals, with a basic resonant frequency of 9 MHz, were modified by a series of surface pretreatment as followings: gold plated

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::25f6b636c592c6406c37e813e8ff8a4b
https://pubmed.ncbi.nlm.nih.gov/2394189

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