Isolation and characterization of the E. coli membrane protein production strain Mutant56(DE3)

Autor: Baumgarten, Thomas, Schlegel, Susan, Wagner, Samuel, Löw, Mirjam, Eriksson, Jonas, Bonde, Ida, Herrgård, Markus J, Heipieper, Hermann J, Nørholm, Morten H H, Slotboom, Dirk Jan, de Gier, Jan-Willem

Publikováno v: Baumgarten, T, Schlegel, S, Wagner, S, Löw, M, Eriksson, J, Bonde, I, Herrgard, M, Heipieper, H J, Nørholm, M, Slotboom, D J & de Gier, J-W 2017, ' Isolation and characterization of the E. coli membrane protein production strain Mutant56(DE3) ', Scientific Reports, vol. 7, 45089 . https://doi.org/10.1038/srep45089
Scientific Reports
Scientific Reports, 7
Scientific Reports, 7:45089, 1-14. Nature Publishing Group

Membrane protein production is usually toxic to E. coli. However, using genetic screens strains can be isolated in which the toxicity of membrane protein production is reduced, thereby improving production yields. Best known examples are the C41(DE3)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e21d8cf574f80684bae5df1b41c08662
https://orbit.dtu.dk/en/publications/77893ed0-10ff-470a-bbd2-47fbe0cd0fd1

Tento výsledek nelze pro nepřihlášené uživatele zobrazit.
K zobrazení výsledku je třeba se přihlásit.

Isolation and characterization of the E. coli membrane protein production strain Mutant56(DE3).

Autor: Baumgarten T; Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, SE-106 91, Sweden., Schlegel S; Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, SE-106 91, Sweden., Wagner S; Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, SE-106 91, Sweden., Löw M; Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, SE-106 91, Sweden., Eriksson J; Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, SE-106 91, Sweden., Bonde I; Technical University of Denmark, Novo Nordisk Foundation Center for Biosustainability, Kogle Alle 6, Hørsholm, 2970, Denmark., Herrgård MJ; Technical University of Denmark, Novo Nordisk Foundation Center for Biosustainability, Kogle Alle 6, Hørsholm, 2970, Denmark., Heipieper HJ; Helmholtz Centre for Environmental Research-UFZ, Department of Environmental Biotechnology, Permoserstrasse 15, Leipzig, 04318, Germany., Nørholm MH; Technical University of Denmark, Novo Nordisk Foundation Center for Biosustainability, Kogle Alle 6, Hørsholm, 2970, Denmark., Slotboom DJ; Groningen Biomolecular Science and Biotechnology Institute, University of Groningen, Nijenborgh 4, Groningen, AG, 9747, The Netherlands., de Gier JW; Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, SE-106 91, Sweden.

Publikováno v: Scientific reports [Sci Rep] 2017 Mar 24; Vol. 7, pp. 45089. Date of Electronic Publication: 2017 Mar 24.