Recombinant OC43 SARS-CoV-2 spike replacement virus: An improved BSL-2 proxy virus for SARS-CoV-2 neutralization assays.

Autor: Hu Z; Cellular Biology Section, Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892., López-Muñoz AD; Cellular Biology Section, Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892., Kosik I; Cellular Biology Section, Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892., Li T; Cellular Biology Section, Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892., Callahan V; Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892., Brooks K; Barrier Immunity Section, Laboratory of Viral Diseases, National Institutes of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892., Yee DS; Barrier Immunity Section, Laboratory of Viral Diseases, National Institutes of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892., Holly J; Cellular Biology Section, Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892., Santos JJS; Cellular Biology Section, Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892., Castro Brant A; Tumor Virus RNA Biology Section, HIV Dynamics and Replication Program, National Cancer Institute, NIH, Frederick, MD 21702., Johnson RF; SARS-CoV-2 Virology Core, Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892., Takeda K; Microscopy and Imaging Core Facility, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD 20993., Zheng ZM; Tumor Virus RNA Biology Section, HIV Dynamics and Replication Program, National Cancer Institute, NIH, Frederick, MD 21702., Brenchley JM; Barrier Immunity Section, Laboratory of Viral Diseases, National Institutes of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892., Yewdell JW; Cellular Biology Section, Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892., Fox JM; Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892.

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Jul 16; Vol. 121 (29), pp. e2310421121. Date of Electronic Publication: 2024 Jul 08.

High-throughput engineering of cytoplasmic- and nuclear-replicating large dsDNA viruses by CRISPR/Cas9.

Autor: López-Muñoz AD; Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, Madrid, Spain.; Present address: Cellular Biology Section, Laboratory of Viral Diseases, NIAID, NIH, Bethesda, Maryland, USA., Rastrojo A; Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, Madrid, Spain.; Present address: Genetic Unit, Department of Biology, Universidad Autónoma de Madrid, Madrid, Spain., Martín R; Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, Madrid, Spain., Alcami A; Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, Madrid, Spain.

Publikováno v: The Journal of general virology [J Gen Virol] 2022 Oct; Vol. 103 (10).

Cell surface SARS-CoV-2 nucleocapsid protein modulates innate and adaptive immunity.

Autor: López-Muñoz AD; Cellular Biology Section, Laboratory of Viral Diseases, NIAID (NIH), Bethesda, MD, USA., Kosik I; Cellular Biology Section, Laboratory of Viral Diseases, NIAID (NIH), Bethesda, MD, USA., Holly J; Cellular Biology Section, Laboratory of Viral Diseases, NIAID (NIH), Bethesda, MD, USA., Yewdell JW; Cellular Biology Section, Laboratory of Viral Diseases, NIAID (NIH), Bethesda, MD, USA.

Publikováno v: Science advances [Sci Adv] 2022 Aug 05; Vol. 8 (31), pp. eabp9770. Date of Electronic Publication: 2022 Aug 03.

Variations in cell-surface ACE2 levels alter direct binding of SARS-CoV-2 Spike protein and viral infectivity: Implications for measuring Spike protein interactions with animal ACE2 orthologs.

Autor: Kazemi S; Department of Biomedical Sciences, Carlson College of Veterinary Medicine, Oregon State University, OR. USA., López-Muñoz AD; Laboratory of Viral Diseases, Cell Biology Section, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda MD. USA., Hollý J; Laboratory of Viral Diseases, Cell Biology Section, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda MD. USA., Jin L; Department of Biomedical Sciences, Carlson College of Veterinary Medicine, Oregon State University, OR. USA., Yewdell JW; Laboratory of Viral Diseases, Cell Biology Section, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda MD. USA., Dolan BP; Department of Biomedical Sciences, Carlson College of Veterinary Medicine, Oregon State University, OR. USA.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2021 Oct 22. Date of Electronic Publication: 2021 Oct 22.

Combination of long- and short-read sequencing fully resolves complex repeats of herpes simplex virus 2 strain MS complete genome.

Autor: López-Muñoz AD; Centro de Biología Molecular Severo Ochoa (Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid), Madrid, Spain.; Present address: Cellular Biology Section, Laboratory of Viral Diseases, NIAID, NIH, Bethesda, MD, USA., Rastrojo A; Centro de Biología Molecular Severo Ochoa (Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid), Madrid, Spain., Kropp KA; Institute of Virology, Hannover Medical School, Hannover, Germany., Viejo-Borbolla A; Institute of Virology, Hannover Medical School, Hannover, Germany., Alcamí A; Centro de Biología Molecular Severo Ochoa (Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid), Madrid, Spain.

Publikováno v: Microbial genomics [Microb Genom] 2021 Jun; Vol. 7 (6).