Crystallization and preliminary X-ray diffraction analysis of selenophosphate synthetases fromTrypanosoma bruceiandLeishmania major

Autor: José Brandão-Neto, Otavio Henrique Thiemann, Marco Túlio Alves da Silva, Marcio Vinicius Bertacine Dias, Lívia Maria Faim, Humberto D'Muniz Pereira, Ivan Rosa e Silva

Publikováno v: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP

Selenophosphate synthetase (SPS) plays an indispensable role in selenium metabolism, being responsible for catalyzing the activation of selenide with adenosine 5'-triphosphate (ATP) to generate selenophosphate, the essential selenium donor for seleno

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8440678f180b549c2fb4f34e0bbe6227
https://doi.org/10.1107/s1744309113014632

Formation of a Ternary Complex for Selenocysteine Biosynthesis in Bacteria

Autor: Daniel M. Saidemberg, Raphaela Makki, Marinônio Lopes Cornélio, Otavio Henrique Thiemann, Lívia Maria Faim, Mario Sergio Palma, Marco Túlio Alves da Silva, Livia Regina Manzine, Ivan Rosa e Silva, Vitor Serrão

Publikováno v: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP

The synthesis of selenocysteine-containing proteins (selenoproteins) involves the interaction of selenocysteine synthase (SelA), tRNA (tRNA(Sec)), selenophosphate synthetase (SelD, SPS), a specific elongation factor (SelB), and a specific mRNA sequen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::896fe9df1bae812033b1d3fe7e4e4bc7
https://pubmed.ncbi.nlm.nih.gov/26378233

The crystal structure of the cysteine protease Xylellain from Xylella fastidiosa reveals an intriguing activation mechanism

Autor: Glaucius Oliva, Maria Amélia Oliva Dotta, Andréia Gianotti, Otavio Henrique Thiemann, Lívia Maria Faim, Flavio Henrique Silva, Ney Ribeiro Leite, Aline Regis Faro

Publikováno v: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP

Xylella fastidiosa is responsible for a wide range of economically important plant diseases. We report here the crystal structure and kinetic data of Xylellain, the first cysteine protease characterized from the genome of the pathogenic X. fastidiosa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed6dcb039e1fc4c9812e358e04937b04
https://pubmed.ncbi.nlm.nih.gov/23333295

Trypanosomatid selenophosphate synthetase structure, function and interaction with selenocysteine lyase.

Autor: da Silva, Marco Túlio Alves¹ (AUTHOR), Silva, Ivan Rosa e¹ (AUTHOR), Faim, Lívia Maria¹ (AUTHOR), Bellini, Natália Karla¹ (AUTHOR), Pereira, Murilo Leão¹ (AUTHOR), Lima, Ana Laura¹ (AUTHOR), de Jesus, Teresa Cristina Leandro^1,2 (AUTHOR), Costa, Fernanda Cristina^1,3 (AUTHOR), Watanabe, Tatiana Faria⁴ (AUTHOR), Pereira, Humberto D'Muniz¹ (AUTHOR), Valentini, Sandro Roberto⁴ (AUTHOR), Zanelli, Cleslei Fernando⁴ (AUTHOR), Borges, Júlio Cesar⁵ (AUTHOR), Dias, Marcio Vinicius Bertacini⁶ (AUTHOR), da Cunha, Júlia Pinheiro Chagas² (AUTHOR), Mittra, Bidyottam⁷ (AUTHOR), Andrews, Norma W.⁷ (AUTHOR), Thiemann, Otavio Henrique^1,8 (AUTHOR) thiemann@ifsc.usp.br

Publikováno v: PLoS Neglected Tropical Diseases. 10/5/2020, Vol. 14 Issue 10, p1-31. 31p.