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Autor:
L. Rodák, Z. Procházka
Publikováno v:
Zentralblatt für Veterinärmedizin Reihe B. 17:924-934
Summary Changes in the lymphoid population of the spleen were followed in chickens immunized at 1, 7 or 21 days of age and injected i/p with 3H-labelled thymidine 1.5 hours prior to antigenic stimulation. The results of these were as follows: 1. In s
Publikováno v:
Veterinary Microbiology. 117:276-283
Astroviral infection has been described as one of the causes of porcine diarrhoeal disease. Here we describe the detection of astrovirus-like particles by electron microscopy in a diarrhoeal specimen. Furthermore, a cytopathic virus was isolated and
Publikováno v:
Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health
Summary Monoclonal antibodies (mAb) to the transmissible gastroenteritis virus (TGEV) nucleoprotein (N) and membrane protein (M) were prepared and used for the comparative assessment of three blocking ELISA variants to detect TGEV. The competitive bl
Publikováno v:
Veterinary Microbiology
Monoclonal antibodies to porcine epidemic diarrhoea virus (PEDV) membrane protein M were prepared and used for the comparative assessment of three blocking ELISA variants to detect PEDV. The competitive blocking ELISA (CB-ELISA) format showed the hig
Publikováno v:
Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health
Summary Monoclonal antibodies to group A rotavirus Vp6 protein were prepared and used for verification of three blocking enzyme‐linked immunosorbent assay (ELISA) modifications to detect rotavirus A. Selected competitive blocking ELISA (CB‐ELISA)
Publikováno v:
Journal of Veterinary Medicine Series B. 50:259-264
Atypical form of myxomatosis, which caused non-lethal and clinically mild disease in domestic rabbits 1 month after immunization with a commercially available vaccine MXT, is described. The isolated myxoma virus designated as Litovel 2 (Li-2) did not
Publikováno v:
Journal of Veterinary Medicine, Series B. 44:425-436
A sensitive and specific ELISA for the demonstration of antibodies to the protein p24 of enzootic bovine leukosis virus (EBVL) using a 'capture' monoclonal antibody to this protein (MAb p24) was developed. The method is sensitive enough to detect the
Publikováno v:
Journal of Fish Diseases. 16:101-111
An enzyme-linked immunosorbent assay (ELISA) for the demonstration of the virus of spring viraemia of carp (SVCV) in liver, kidney and spleen homogenates, and in infected cell cultures is described. The sensitivity of the method is 102·8–103·5 TC
Publikováno v:
Veterinary journal (London, England : 1997). 187(2)
Analysis of 476 faecal samples from diarrhoeic piglets was performed using electron microscopy (EM) and a competitive blocking (CB)-ELISA based on monoclonal antibodies to the VP6 protein of group A rotavirus. Rotavirus was detected by EM and/or CB-E