Zobrazeno 1 - 10
of 37
pro vyhledávání: '"L, Cabanié"'
Publikováno v:
Biotechnology and Bioengineering. 44:753-764
During long-term continuous culture of the hybridoma cell line 11317, a better-producing subclone (I1317-SF11), giving improved productivity, has been selected. The comparison of the original cell line (I1317-DC) with this subclone revealed that alth
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::771242d4cf6a174450cd7f36a56a0679
https://doi.org/10.1002/bit.260440612
https://doi.org/10.1002/bit.260440612
Publikováno v:
Journal of Receptor Research. 13:541-558
A novel screening assay for the identification of baculovirus infected cells expressing membrane receptors was developed by using a replica transfer technique. Sf9 cells were cotransfected with wild type baculoviral DNA and the transfer vector pVL941
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5ea1d9ae71633a41037e68bf19172663
https://doi.org/10.3109/10799899309073678
https://doi.org/10.3109/10799899309073678
Publikováno v:
Biotechnology and bioengineering. 44(6)
During long-term continuous culture of the hybridoma cell line 11317, a better-producing subclone (I1317-SF11), giving improved productivity, has been selected. The comparison of the original cell line (I1317-DC) with this subclone revealed that alth
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::36b78cd7e8fa300dd46c5bc7706c2f80
https://pubmed.ncbi.nlm.nih.gov/18618836
https://pubmed.ncbi.nlm.nih.gov/18618836
The objective of this study was to compare the kinetic characteristics of some key components of monoclonal antibody synthesis (growth, cell specific productivity, mRNAs coding for heavy and light chain IgG) in hybridoma continuous cultures in a prot
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bac5e16259569564b474761085f9adcb
https://doi.org/10.1016/b978-0-7506-1845-8.50122-8
https://doi.org/10.1016/b978-0-7506-1845-8.50122-8
Kinetic characteristics of some key components of monoclonal antibody (MAb) synthesis were compared during hybridoma batch and semicontinuous cultures. During batch culture, there was a general trend of decrease in the amounts of total cellular RNA,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::72dafea958ffbf23d0f35ccd253f7472
https://doi.org/10.1016/b978-0-7506-0421-5.50022-2
https://doi.org/10.1016/b978-0-7506-0421-5.50022-2
The release of lactate dehydrogenase (LDH), alkaline phosphatase (APH), and adenylate kinase (AK) in the culture medium can be used to estimate the dead cell number in cell cultures, whereby satisfactory results have been obtained. Although the cellu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2ed84a074c5bf6bef05b22c77e1f5532
https://doi.org/10.1016/b978-0-7506-0421-5.50079-9
https://doi.org/10.1016/b978-0-7506-0421-5.50079-9
Protein-free media for cultures of mouse hybridoma cell lines in stirred tank reactors have been developed. Starting from a serum-free medium (Merten et al. (1986) 9th ESACT-meeting, Knokke/B), which contained transferrin as the only protein (MEM + a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7c17075046b4c520f876d1d0f3fda7d8
https://doi.org/10.1016/b978-0-7506-1103-9.50043-3
https://doi.org/10.1016/b978-0-7506-1103-9.50043-3
Pulse-experiments, avoiding changes in the cellular physiology, have been conducted, in order to establish, (a) wether the secretion of monoclonal antibodies (mAbs) by mouse hybridomas occurs mainly by a de novo synthesis or by a release of intracell
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c1803fa0c1dc1ef70d908dcff5946311
https://doi.org/10.1016/b978-0-7506-1103-9.50113-x
https://doi.org/10.1016/b978-0-7506-1103-9.50113-x
Publikováno v:
Cytotechnology. 4(1)
Pulse chase experiments of two mouse hybridoma lines were conducted in order to elucidate the kinetics of monoclonal antibody (mAb) production and secretion during different stages of batch cultures. The results indicate that a stock of cytoplasmic I
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c619d5a23a7dde70353e60030ef5542
https://pubmed.ncbi.nlm.nih.gov/1366724
https://pubmed.ncbi.nlm.nih.gov/1366724
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