Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Kyoko Saotome"'
Autor:
Makoto Hayashi, Kyoko Saotome
Publikováno v:
Mutagenesis. 18:73-76
We have improved our sea urchin micronucleus assay for aquatic samples and used it to evaluate marine pollution. We found that the water samples we had collected for 2 years from the Tokyo bay coast near Tokyo, an industrial megalopolis, were positiv
Autor:
Kyoko Saotome, Yoshio Ojima
Publikováno v:
Zoological Science. 18:683-686
We characterized the chromosomes of the lancelet Branchiostoma belcheri Gray. Testes were removed from adult males, cut up, treated with colchicine (25–50 μg/ml) for 3–6 hr, and dissociated into single cells by pippeting. The dissociated cells w
Publikováno v:
Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 446:121-127
We have developed a micronucleus assay for use in sea urchin embryos. The embryos at the early blastula stage (about 256 cells) were exposed to genotoxic chemicals overnight until control embryos have reached the gastrula stage. Then all embryos were
Autor:
Kyoko Saotome
Publikováno v:
Zoological Science. 16:87-92
The chromosome number of the andromerogones obtained by fertilizing non-nucleate egg fragments of Hemicentrotus pulcherrimus was examined by an air-drying method during early development. The non-nucleate egg fragments were prepared by centrifuging u
Autor:
Emi Nishidate, Naonori Matsusaka, Shuji Tsuda, YūF. Sasaki, Norihide Asano, Kyoko Saotome, Satoko Ishibashi, Toshio Sofuni, Makoto Hayashi, Fusako Izumiyama
Publikováno v:
Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 393:133-139
We exposed two species of shellfish, Patunopecten yessoensis and Tapes japonica , for 4 h to artificial sea water in which N -methyl- N ′-nitro- N -nitrosoguanidine (MNNG), ethyl nitrosourea (EMS), 3-chloro-4-dichloromethyl-5-hydroxy-2( H )-furanon
Publikováno v:
Gynecologic and Obstetric Investigation. 42:206-208
Turner's syndrome associated with an X ring chromosome, r (X), is rare and there has been no report on pregnancy in Turner's syndrome with 45,X/46,Xr (X)/46,XX mosaicism. A patient with this karyotype who lacked the clinical manifestation of characte
Autor:
Miéko Komatsu, Kyoko Saotome
Publikováno v:
Zoological science. 19(10)
We developed a method for preparing starfish chromosomes from embryos. Blastulae were treated with colchicine (0.2–4.0 mg/ml), dissociated into single blastomeres by pippeting, swollen with 7% sodium citrate, and fixed with methanol: acetic acid (3
Publikováno v:
Zoological science. 19(2)
We developed a method for preparing male chromosomes from sea urchin hybrid andromerogones created with cryopreserved sperm. We obtained hybrid andromerogones by heterospermic insemination of Hemicentrotus pulcherrimus non-nucleate egg fragments prod
Autor:
Kyoko Saotome, Tomio Yanagisawa
Publikováno v:
Development, Growth and Differentiation. 21:413-421
Autor:
Tomio Yanagisawa, Kyoko Saotome
Publikováno v:
Development, Growth and Differentiation. 21:401-411
Sulfated polysaccharides produced by the reaction which transfers sulfate from 3′-phosphoadenosine 5′-phosphosulfate to endogenous acceptors in sea urchin embryos were characterized by their precipitability with cetylpyridinium chloride, chromato