Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Kyoko Aikawa"'
Publikováno v:
The Journal of Chemical Physics. 158:134510
The vibrational dynamics of the NO stretching mode of [RuCl5(NO)]2− in D2O were investigated by nonlinear infrared (IR) spectroscopy. We performed IR pump–probe measurements to obtain the vibrational lifetime of this molecule. The lifetime is 31
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::cb41697ddd987f37ee5bde2f09050307
https://doi.org/10.1063/5.0139133
https://doi.org/10.1063/5.0139133
This protocol offers an safer alternative to the ethidium bromide-based DNA quantification protocol, utilizing an DNA-selective dye EzFluoroStain DNA (WSE-7130, ATTO corporation, Tokyo, Japan). This protocol allows to quantify about 2-1000 ng DNA/wel
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c66d7c67e672424d1053fa2b9b94a363
https://doi.org/10.17504/protocols.io.bztvp6n6
https://doi.org/10.17504/protocols.io.bztvp6n6
Autor:
Rina Naradate, Yusuke Suzuki, Chihiro Tomita, Haruko Ogawa, Nanami Kawaguchi, Kyoko Aikawa, Tomoko Higami, Kimie Date, Kosuke Nakamura
Publikováno v:
Journal of Biochemistry
An active recombinant human pancreatic lipase (recHPL) was successfully prepared for the first time from the Escherichia coli expression system using short Strep-tag II (ST II). The recHPL–ST II was solubilized using 8 M urea from E.coli lysate and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c71657d6299c6b9d7cc229813e33fb4
http://europepmc.org/articles/PMC6267337
http://europepmc.org/articles/PMC6267337
Publikováno v:
Water
Volume 11
Issue 10
Water, Vol 11, Iss 10, p 2156 (2019)
Volume 11
Issue 10
Water, Vol 11, Iss 10, p 2156 (2019)
This study investigated how chlorine inactivates and damages Escherichia coli cells. E. coli that had transformed to express enhanced green fluorescent protein (EGFP) at the cytoplasm was treated with chlorine. Damage to the cell membrane and cell wa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b2f3327f6a076005c8b2ce59c8224648
Autor:
Hiroshi Jinmei, Kyoko Aikawa, Hiroshi Abe, Kazuhiro Furukawa, Isamu Matsumoto, Naoko Abe, Satoshi Tsuneda, Atsushi Uchiyama, Yoshihiro Ito, Yuko Kondo
Publikováno v:
Bioconjugate Chemistry. 19:327-333
Enzymatic ligation methods are useful in the diagnostic detection of DNA sequences. Here, we describe the investigation of nonenzymatic phosphorothioate--iodoacetyl DNA chemical ligation as a method for the detection and identification of RNA and DNA
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::908a7cf833bc9394bf64ccc4080be2fb
https://doi.org/10.1021/bc700244s
https://doi.org/10.1021/bc700244s
Publikováno v:
Springer Proceedings in Physics ISBN: 9783319132419
Vibrational dynamics of nitrosyl stretching mode of [RuCl5(NO)]2− in water was studied by two-dimensional infrared spectroscopy. Observed temperature dependence of the correlation function of vibrational frequency fluctuation clearly shows that the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::70b5a1464c5c13e0ccb3ea3f95495555
https://doi.org/10.1364/up.2014.07.mon.e.6
https://doi.org/10.1364/up.2014.07.mon.e.6
Publikováno v:
Colloids and Surfaces A: Physicochemical and Engineering Aspects. 150:95-104
Mesoporous silica particles have been prepared by hydrolysis of TEOS (Si(OC2H5)4) in bicontinuous microemulsions containing polyoxyethylene (POE) dodecylether, isooctane and water. TEOS was dissolved in a continuous water phase and hydrolyzed by the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::21162dd07f5aa5b76ca781f2d63a282e
https://doi.org/10.1016/s0927-7757(98)00718-3
https://doi.org/10.1016/s0927-7757(98)00718-3
Publikováno v:
Chemical communications (Cambridge, England). (43)
We have developed an S(N)Ar reaction-triggered fluorescence probe using a new fluorogenic compound derivatized from 7-aminocoumarin for oligonucleotides detection.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b1570d838c61df0a4e5f153d6439e057
https://pubmed.ncbi.nlm.nih.gov/19865658
https://pubmed.ncbi.nlm.nih.gov/19865658
Autor:
Isamu Matsumoto, Hiroshi Abe, Naoko Abe, Yoshihiro Ito, Hiroshi Jinmei, Kyoko Aikawa, Yuko Kondo
Publikováno v:
Nucleic Acids Symposium Series. 51:353-354
Enzymatic ligation methods are useful in diagnostic detection of DNA sequence. Here we describe the investigation of nonezymatic phosphorothioate-iodoacetyl DNA chemical ligation as a method for detection and identification of RNA and DNA. Specificit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fa9b085a03d5be0c22551e817bb9e4ce
https://doi.org/10.1093/nass/nrm177
https://doi.org/10.1093/nass/nrm177
Publikováno v:
Chemical Communications. 47:8367
Branched RNAs with three- or four-way junctions were designed by assembling single-stranded RNA for RNA interference. Human Dicer transformed branched RNAs into about 20 base pairs of double-stranded RNA, which is a standard siRNA species. Our tetram
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7019f3701414c1a61bffde81571952e
https://doi.org/10.1039/c1cc11780g
https://doi.org/10.1039/c1cc11780g