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pro vyhledávání: '"Kyohei Horio"'
Autor:
Kyohei Horio, Hirokazu Takahashi, Toshiro Kobori, Kenshi Watanabe, Tsunehiro Aki, Yutaka Nakashimada, Yoshiko Okamura
Publikováno v:
Microorganisms, Vol 9, Iss 6, p 1208 (2021)
Recently, we developed an in situ mRNA detection method termed RNase H-assisted rolling circle amplification-fluorescence in situ hybridization (RHa-RCA-FISH), which can detect even short mRNA in a bacterial cell. However, because this FISH method is
Externí odkaz:
https://doaj.org/article/c502bd22e4b04cf6b5ecc0552a2981da
Autor:
Tsunehiro Aki, Hirokazu Takahashi, Yutaka Nakashimada, Kenshi Watanabe, Toshiro Kobori, Kyohei Horio, Yoshiko Okamura
Publikováno v:
Microorganisms, Vol 9, Iss 1208, p 1208 (2021)
Microorganisms
Volume 9
Issue 6
Microorganisms
Volume 9
Issue 6
Recently, we developed an in situ mRNA detection method termed RNase H-assisted rolling circle amplification-fluorescence in situ hybridization (RHa-RCA-FISH), which can detect even short mRNA in a bacterial cell. However, because this FISH method is
Autor:
Kyohei Horio, Yoshiko Okamura, Hirokazu Takahashi, Toshiro Kobori, Tsunehiro Aki, Yutaka Nakashimada, Kenshi Watanabe, Setsu Kato
Publikováno v:
Scientific Reports
Scientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)
Scientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)
Meta-analyses using next generation sequencing is a powerful strategy for studying microbiota; however, it cannot clarify the role of individual microbes within microbiota. To know which cell expresses what gene is important for elucidation of the in
Autor:
Kyohei Horio, Toshiro Kobori, Yukihiko Matsumura, Yutaka Nakashimada, Masahiko Ohkawachi, Tsunehiro Aki, Hirokazu Takahashi, Yoshiko Okamura
Publikováno v:
Scientific Reports
Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
SC30201903070009
NARO成果DBa
Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
SC30201903070009
NARO成果DBa
RNA-primed rolling circle amplification (RPRCA) is a useful laboratory method for RNA detection; however, the detection of RNA is limited by the lack of information on 3′-terminal sequences. We uncovered that conventional RPRCA using pre-circulariz