Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Kyle J. Seamon"'
Autor:
Junru Cui, James T. Stivers, Anthony S. Gizzi, Matthew Egleston, Benjamin Orris, Eric S. Christenson, Michael DePasquale, Kyle J. Seamon, Ben Ho Park
Publikováno v:
Molecular Pharmacology. 99:412-425
Previous short-hairpin RNA knockdown studies have established that depletion of human uracil DNA glycosylase (hUNG) sensitizes some cell lines to 5-fluorodeoxyuridine (FdU). Here, we selectively inhibit the catalytic activity of hUNG by lentiviral tr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::769bc1ef00d9adc530b4cc371ed8abdb
https://doi.org/10.1124/molpharm.120.000191
https://doi.org/10.1124/molpharm.120.000191
Autor:
Christopher J. Schulze, Jim Cregg, Kyle J. Seamon, Yu Chi Yang, Zhican Wang, Lindsay S. Garrenton, Alun Bermingham, John E. Knox, Aidan Tomlinson, Kang-Jye Chou, Shaoling Li, David P. Wildes, Mallika Singh, Elena S. Koltun, Adrian L. Gill, Robert J. Nichols, Jacqueline A. Smith
Publikováno v:
Cancer Research. 82:3598-3598
The clinical activity of KRASG12C(OFF) inhibitors has validated KRASG12C as an oncogenic driver in human cancers and demonstrated the power of targeting cysteine mutations with covalent inhibitors. A second oncogenic RAS cysteine mutation, KRASG13C,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a391fa7cfc38c6ad08db316050b88ca3
https://doi.org/10.1158/1538-7445.am2022-3598
https://doi.org/10.1158/1538-7445.am2022-3598
Autor:
Robert J. Meagher, Anchal Sinha, Brooke Harmon, Joseph S. Schoeniger, Tyler P. Eckles, Christopher R. Phaneuf, Kyle J. Seamon, Vinay V. Abhyankar, Chung-Yan Koh
Publikováno v:
Analytical Methods. 11:559-565
The discovery of the RNA-guided DNA nuclease CRISPR-Cas9 has enabled the targeted editing of genomes from diverse organisms, but the permanent and inheritable nature of genome modification also poses immense risks. The potential for accidental exposu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e9eed3f61960a09faf7b56be1dbfc39a
https://doi.org/10.1039/c8ay02726a
https://doi.org/10.1039/c8ay02726a
Publikováno v:
Analytical Chemistry. 90:6913-6921
The RNA-guided DNA nuclease Cas9 is now widely used for the targeted modification of genomes of human cells and various organisms. Despite the extensive use of Clustered Regularly Interspaced Palindromic Repeats (CRISPR) systems for genome engineerin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::15046b82daf5ff3894e165ac9e51bdcb
https://doi.org/10.1021/acs.analchem.8b01155
https://doi.org/10.1021/acs.analchem.8b01155
Autor:
Jason C. Harper, Edwin A. Saada, Joshua D. Podlevsky, Steven S. Branda, Robert K. Johnston, Jerilyn A. Timlin, Kyle J. Seamon
Publikováno v:
Biosensorsbioelectronics. 141
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) ribonucleoprotein (RNP) complex is an RNA-guided DNA-nuclease that is part of the bacterial adaptive immune system. CRISPR/Cas9 RNP has been ada
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d77917de209c75fe621e8b048abfa658
https://pubmed.ncbi.nlm.nih.gov/31207570
https://pubmed.ncbi.nlm.nih.gov/31207570
Publikováno v:
Nucleic Acids Research
The HIV-1 restriction factor SAMHD1 is a tetrameric enzyme activated by guanine nucleotides with dNTP triphosphate hydrolase activity (dNTPase). In addition to this established activity, there have been a series of conflicting reports as to whether t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1bd734900cec8bda0b0bcd2f878da68
https://doi.org/10.1093/nar/gkv633
https://doi.org/10.1093/nar/gkv633
Autor:
Namandjé N. Bumpus, Kyle J. Seamon, Anastasia P. Kadina, James T. Stivers, Erik C Hansen, Boris A. Kashemirov, Charles E. McKenna
Publikováno v:
Journal of the American Chemical Society
SAMHD1 is a GTP-activated nonspecific dNTP triphosphohydrolase that depletes dNTP pools in resting CD4+ T cells and macrophages and effectively restricts infection by HIV-1. We have designed a nonsubstrate dUTP analogue with a methylene bridge connec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d97b6330ddd51f8bf702b6333805e713
https://doi.org/10.1021/ja5035717
https://doi.org/10.1021/ja5035717
Publikováno v:
Biochemistry. 55(44)
Sterile alpha motif and HD domain protein 1 (SAMHD1) is a unique enzyme that plays important roles in nucleic acid metabolism, viral restriction, and the pathogenesis of autoimmune diseases and cancer. Although much attention has been focused on its
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::637ad6a59a117a14479ad5c32df32caa
https://pubmed.ncbi.nlm.nih.gov/27775344
https://pubmed.ncbi.nlm.nih.gov/27775344
Publikováno v:
The FASEB Journal. 26:1506-1516
Estrogen receptors (ERs) are expressed in leukocytes and in every ocular tissue. However, sex-specific differences and the role of estradiol in ocular inflammatory-reparative responses are not well understood. We found that female mice exhibited dela
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e0ebdd2f8c46affa55b6a6b3637e3581
https://doi.org/10.1096/fj.11-198036
https://doi.org/10.1096/fj.11-198036
Autor:
James T. Stivers, Kyle J. Seamon
Sterile alpha motif and histidine-aspartate domain-containing protein 1 (SAMHD1) is a recently discovered enzyme that plays a central role in nucleotide metabolism and innate immunity. SAMHD1 has deoxyribonucleoside triphosphate (dNTP) triphosphohydr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ee152e43823df8506e098a0d1b35c54
https://europepmc.org/articles/PMC4475474/
https://europepmc.org/articles/PMC4475474/
