Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Kye Chesnut"'
Publikováno v:
Gene Therapy. 7:1783-1789
The conducting airways are the primary target for gene transfer in cystic fibrosis (CF), yet the inflammation associated with CF lung disease could potentially pose a significant barrier to gene transfer vectors, such as recombinant adeno-associated
Autor:
Terence R. Flotte, William B. Guggino, Suzanne E. Beck, Thomas C. Reynolds, Lori A. Jones, Scott Walsh, Kye Chesnut, Barrie J. Carter, Frederic B. Askin
Publikováno v:
Journal of Virology. 73:9446-9455
Efficient local expression from recombinant adeno-associated virus (rAAV)-cystic fibrosis (CF) transmembrane conductance regulator (CFTR) vectors has been observed in the airways of rabbits and monkeys for up to 6 months following a single bronchosco
Autor:
Barry J. Byrne, Mark Potter, C Summerford, Richard Jude Samulski, Nicholas Muzyczka, Sergei Zolotukhin, Kye Chesnut, I Zolotukhin, E Mason
Publikováno v:
Gene Therapy. 6:973-985
Conventional methods for rAAV purification that are based on cesium chloride ultracentrifugation have often produced vector preparations of variable quality and resulted in significant loss of particle infectivity. We report here several novel purifi
Publikováno v:
Gene Therapy. 5:166-173
The green fluorescent protein (GFP) has previously been adapted as a reported for gene transfer and expression in mammalian cells in culture and in tissue sections. Herein is described a new method for detecting GFP in situ within epithelia accessibl
Characterizations of candidate genes for IDD susceptibility from the diabetes-prone NOD mouse strain
Publikováno v:
Mammalian Genome. 4:549-554
The nucleotide sequences of the NOD and C57BL/6J alleles of Glut-2, Sod-2, and Il-2 were determined by RT-PCR sequencing. Each of these loci is located in intervals that strongly correlated with susceptibility to diabetes in an (NOD/Uf x C57BL/6J)F1
Autor:
Sihong Song, Kevin D. Foust, Thomas J. Conlon, Terence R. Flotte, Kye Chesnut, Scott A. Loiler, Benjamin D Zusman, Thomas W. Ferkol, Isabel Virella-Lowell
Publikováno v:
The journal of gene medicine. 7(7)
Despite favorable DNA transfer efficiency, gene expression from recombinant adeno-associated virus (rAAV2) vectors in the lung has been variable in the context of cystic fibrosis (CF) gene therapy. This is due, in part, to the large size of the CF tr
Autor:
Terence R. Flotte, Kye Chesnut, Anne C. Fischer, Robert J. Adams, William B. Guggino, Carolina I. Barberena, Beth L. Laube, Suzanne E. Beck
Publikováno v:
Molecular therapy : the journal of the American Society of Gene Therapy. 6(4)
The goals of these experiments were to efficiently deliver aerosolized adeno-associated virus (AAV) vector to the lungs of Rhesus macaques and to measure gene transfer and expression. To determine optimal lung deposition, we compared four techniques
Autor:
Anupam Agarwal, Sihong Song, Luca Inverardi, Kye Chesnut, Harry S. Nick, Sandra Afione, Matthias H. Kapturczak, Tamir M. Ellis, Jianming Wang, Elizabeth S. Fenjves, Mark A. Atkinson, Terence R. Flotte, Clive Wasserfall, Scott A. Loiler
Publikováno v:
Diabetes. 50(3)
The ability to transfer immunoregulatory, cytoprotective, or antiapoptotic genes into pancreatic islet cells may allow enhanced posttransplantation survival of islet allografts and inhibition of recurrent autoimmune destruction of these cells in type