Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Kwok-Kam Yeung"'
Publikováno v:
Biochemical and Biophysical Research Communications. 59:252-260
Summary The activities of four glycolipid glycosyltransferases involved in the biosynthesis in vitro of globoside and Forssman hapten were measured in normal mouse adrenal tissues, Y-1 mouse adrenal tumor cells, and tumors derived therefrom. These en
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a52da47cf6a222cebe462c86cb400f08
https://doi.org/10.1016/s0006-291x(74)80200-7
https://doi.org/10.1016/s0006-291x(74)80200-7
Publikováno v:
Journal of Neurochemistry. 35:407-411
An extract of glycosidic enzymes from young rat brain was immobilized to cyanogen bromide-activated Sepharose 4B. Most glycosidases retained approximately 10--25% of their activities after immobilization. Immobilized beta-galactosidases were used rep
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c894ffb454ee3bf3b19e1dca4339c322
https://doi.org/10.1111/j.1471-4159.1980.tb06279.x
https://doi.org/10.1111/j.1471-4159.1980.tb06279.x
Autor:
Robert C. Boguslaski, Robert J. Carrico, James Edward Christner, Robert T. Buckler, Hartmut R. Schroeder, Kwok-Kam Yeung
Publikováno v:
Analytical Biochemistry. 76:95-110
A new method was developed to monitor specific protein binding reactions with an ATP-labeled ligand and firefly luciferase. The ligand, 2,4-dinitrobenzene, was covalently coupled to four ATP derivatives and three of these conjugates were measured qua
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f73e0194661530132a5cca23d1ecea43
https://doi.org/10.1016/0003-2697(76)90267-0
https://doi.org/10.1016/0003-2697(76)90267-0
Publikováno v:
Analytical Biochemistry. 72:271-282
Specific binding reactions were monitored by a new enzymatic method which employs cofactor labeled ligands. The ligands biotin and 2,4-dinitrofluorobenzene were coupled covalently to a derivative of NAD, nicotinamide 6-(2-aminoethylamino) purine dinu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b1e91c4a07ecacb1d9911102166c325d
https://doi.org/10.1016/0003-2697(76)90530-3
https://doi.org/10.1016/0003-2697(76)90530-3
Publikováno v:
Carbohydrate research. 75
A mixture of glycosidases from the liver of the gastropod Turbo cornutus was co-immobilized with bovine serum albumin and glutaraldehyde, and then cast as membranes. The properties of immobilized N-acetyl-beta-D-hexosaminidase were studied. The recov
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::add16ccdaaa404b694f764db847b5744
https://pubmed.ncbi.nlm.nih.gov/39677
https://pubmed.ncbi.nlm.nih.gov/39677
Publikováno v:
Advances in Experimental Medicine and Biology ISBN: 9781461590736
A mixture of glycosidases from the liver of sea worm T. cornutus was co-immobilized with bovine serum albumin and glutaraldehyde and then cast as membranes. When the activities of nine glycosidases were examined, it was found that only a - mannosidas
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bcc54d0a96822b5405f84e312ae41824
https://doi.org/10.1007/978-1-4615-9071-2_50
https://doi.org/10.1007/978-1-4615-9071-2_50
Autor:
Kwok Kam Yeung, Robert J. Carrico
Publikováno v:
Analytical biochemistry. 74(2)
A simple and rapid method for the purification of NADP-dependent malic enzyme from chicken liver is described. A crude tissue extract was chromatographed on an affinity column containing immobilized N6-(6-aminohexyl)-adenosine 2′,5′-bisphosphate.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::69e60dbda8eb89d86ed491a9976b83d2
https://pubmed.ncbi.nlm.nih.gov/9010
https://pubmed.ncbi.nlm.nih.gov/9010
Publikováno v:
Comparative biochemistry and physiology. B, Comparative biochemistry. 76(3)
1. 1. β-Galactosidase from T. cornutus was resolved into two activity peaks by gel filtration column chromatography. 2. 2. The pH optima of the two peaks designated P1 and P2, were 5.5 and 3.0, respectively, when p- nitrophenyl -β- d -galactopyrano
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e75ad52433fdac94475e5c3cbdbfb656
https://pubmed.ncbi.nlm.nih.gov/6416742
https://pubmed.ncbi.nlm.nih.gov/6416742
Publikováno v:
Analytical biochemistry. 94(1)
A fluorometric method for monitoring the enzymic hydrolysis of the terminal galactose from GM 1 -ganglioside has been developed. The released galactose is oxidized with galactose dehydrogenase and NAD and the fluorescence of the product NADH measured
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f1b06b1c000dc55f8f4a35d18ebbff3e
https://pubmed.ncbi.nlm.nih.gov/223469
https://pubmed.ncbi.nlm.nih.gov/223469
Publikováno v:
Comparative biochemistry and physiology. B, Comparative biochemistry. 63(3)
1. 1. β - N -Acetylhexosaminidase isoenzymes from the gastropod, T. cornutus , were purified and their properties studied. 2. 2. The two isoenzymes, designated A and B were separated by DEAE-Sephadex column chromatography and further purified by CM-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::075cff98ede86116363a9da3cfe1bfa2
https://pubmed.ncbi.nlm.nih.gov/162585
https://pubmed.ncbi.nlm.nih.gov/162585