pKID Binds to KIX via an Unstructured Transition State with Nonnative Interactions

Autor: Dahal, L, Kwan, TOC, Shammas, SL, Clarke, J

Publikováno v: Biophysical Journal

Understanding the detailed mechanism of interaction of intrinsically disordered proteins with their partners is crucial to comprehend their functions in signaling and transcription. Through its interaction with KIX, the disordered pKID region of CREB

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::5f1e822f5fde929ec604a1938747c6e7
https://www.repository.cam.ac.uk/handle/1810/276397

A versatile approach to high-density microcrystals in lipidic cubic phase for room-temperature serial crystallography.

Autor: Birch J; Membrane Protein Laboratory, Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0DE, United Kingdom.; Research Complex at Harwell, Rutherford Appleton Laboratory, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0FA, United Kingdom., Kwan TOC; ChemBio, National Physical Laboratory, Hampton Road, Teddington, Middlesex TW11 0LW, United Kingdom., Judge PJ; Biochemistry Department, Oxford University, South Parks Road, Oxford OX1 3QU, United Kingdom., Axford D; Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0DE, United Kingdom., Aller P; Research Complex at Harwell, Rutherford Appleton Laboratory, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0FA, United Kingdom.; Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0DE, United Kingdom., Butryn A; Research Complex at Harwell, Rutherford Appleton Laboratory, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0FA, United Kingdom.; Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0DE, United Kingdom., Reis RI; ChemBio, National Physical Laboratory, Hampton Road, Teddington, Middlesex TW11 0LW, United Kingdom., Bada Juarez JF; Biochemistry Department, Oxford University, South Parks Road, Oxford OX1 3QU, United Kingdom.; Ecole Polytechnique Fédérale de Lausanne (EPFL), Station 19, Lausanne, CH-1015, Switzerland., Vinals J; Biochemistry Department, Oxford University, South Parks Road, Oxford OX1 3QU, United Kingdom.; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA., Owen RL; Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0DE, United Kingdom., Nango E; RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo, 679-5148, Japan.; Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, 980-8577, Japan., Tanaka R; RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo, 679-5148, Japan.; Department of Cell Biology, Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho, Sakyo-ku, Kyoto, 606-8501, Japan., Tono K; RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo, 679-5148, Japan.; Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo, 679-5148, Japan., Joti Y; RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo, 679-5148, Japan.; Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo, 679-5148, Japan., Tanaka T; RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo, 679-5148, Japan.; Department of Cell Biology, Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho, Sakyo-ku, Kyoto, 606-8501, Japan., Owada S; RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo, 679-5148, Japan.; Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo, 679-5148, Japan., Sugahara M; RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo, 679-5148, Japan., Iwata S; RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo, 679-5148, Japan.; Department of Cell Biology, Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho, Sakyo-ku, Kyoto, 606-8501, Japan., Orville AM; Research Complex at Harwell, Rutherford Appleton Laboratory, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0FA, United Kingdom.; Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0DE, United Kingdom., Watts A; Biochemistry Department, Oxford University, South Parks Road, Oxford OX1 3QU, United Kingdom., Moraes I; ChemBio, National Physical Laboratory, Hampton Road, Teddington, Middlesex TW11 0LW, United Kingdom.

Publikováno v: Journal of applied crystallography [J Appl Crystallogr] 2023 Aug 18; Vol. 56 (Pt 5), pp. 1361-1370. Date of Electronic Publication: 2023 Aug 18 (Print Publication: 2023).

Measuring Protein Aggregation and Stability Using High-Throughput Biophysical Approaches.

Autor: Kwan TOC; National Physical Laboratory, Teddington, United Kingdom., Kolek SA; Douglas Instruments Ltd., Hungerford, United Kingdom., Danson AE; National Physical Laboratory, Teddington, United Kingdom., Reis RI; National Physical Laboratory, Teddington, United Kingdom., Camacho IS; National Physical Laboratory, Teddington, United Kingdom., Shaw Stewart PD; Douglas Instruments Ltd., Hungerford, United Kingdom., Moraes I; National Physical Laboratory, Teddington, United Kingdom.

Publikováno v: Frontiers in molecular biosciences [Front Mol Biosci] 2022 May 16; Vol. 9, pp. 890862. Date of Electronic Publication: 2022 May 16 (Print Publication: 2022).

Structures of the archaerhodopsin-3 transporter reveal that disordering of internal water networks underpins receptor sensitization.

Autor: Bada Juarez JF; Biochemistry Department, Oxford University, South Parks Road, Oxford, OX1 3QU, UK., Judge PJ; Biochemistry Department, Oxford University, South Parks Road, Oxford, OX1 3QU, UK., Adam S; Fritz Haber Center for Molecular Dynamics Research, Institute of Chemistry, Hebrew University of Jerusalem, Jerusalem, 9190401, Israel., Axford D; Diamond Light Source, Harwell Science and Innovation Campus, Didcot, OX11 0DE, UK., Vinals J; Biochemistry Department, Oxford University, South Parks Road, Oxford, OX1 3QU, UK., Birch J; Diamond Light Source, Harwell Science and Innovation Campus, Didcot, OX11 0DE, UK.; Research Complex at Harwell, Rutherford Appleton Laboratory, Harwell Science and Innovation Campus, Didcot, OX11 0FA, UK., Kwan TOC; Research Complex at Harwell, Rutherford Appleton Laboratory, Harwell Science and Innovation Campus, Didcot, OX11 0FA, UK.; National Physical Laboratory, Hampton Road, Teddington, London, TW11 0LW, UK., Hoi KK; Chemistry Research Laboratory, Oxford University, Mansfield Road, Oxford, OX1 3TA, UK., Yen HY; OMass Therapeutics, The Schrodinger Building, Oxford Science Park, Oxford, OX4 4GE, UK., Vial A; Centre de Biochimie Structurale (CBS), INSERM, CNRS, University of Montpellier, Montpellier, France., Milhiet PE; Centre de Biochimie Structurale (CBS), INSERM, CNRS, University of Montpellier, Montpellier, France., Robinson CV; Chemistry Research Laboratory, Oxford University, Mansfield Road, Oxford, OX1 3TA, UK., Schapiro I; Fritz Haber Center for Molecular Dynamics Research, Institute of Chemistry, Hebrew University of Jerusalem, Jerusalem, 9190401, Israel., Moraes I; Research Complex at Harwell, Rutherford Appleton Laboratory, Harwell Science and Innovation Campus, Didcot, OX11 0FA, UK. isabel.moraes@npl.co.uk.; National Physical Laboratory, Hampton Road, Teddington, London, TW11 0LW, UK. isabel.moraes@npl.co.uk., Watts A; Biochemistry Department, Oxford University, South Parks Road, Oxford, OX1 3QU, UK. anthony.watts@bioch.ox.ac.uk.

Publikováno v: Nature communications [Nat Commun] 2021 Jan 27; Vol. 12 (1), pp. 629. Date of Electronic Publication: 2021 Jan 27.

Membrane protein crystallography in the era of modern structural biology.

Autor: Kwan TOC; National Physical Laboratory, Hampton Road, Teddington TW11 0LW, U.K.; Research Complex at Harwell, Rutherford Appleton Laboratory, Harwell Science and Innovation Campus, Didcot OX11 0FA, U.K., Axford D; Diamond Light Source, Harwell Science and Innovation Campus, Oxfordshire OX11 0DE, U.K., Moraes I; National Physical Laboratory, Hampton Road, Teddington TW11 0LW, U.K.; Research Complex at Harwell, Rutherford Appleton Laboratory, Harwell Science and Innovation Campus, Didcot OX11 0FA, U.K.

Publikováno v: Biochemical Society transactions [Biochem Soc Trans] 2020 Dec 18; Vol. 48 (6), pp. 2505-2524.