Zobrazeno 1 - 10
of 247
pro vyhledávání: '"Kurt. V. Gothelf"'
Autor:
Laura Teodori, Sarah K. Ochoa, Marjan Omer, Veronica L. Andersen, Pernille Bech, Junyi Su, Jessica Bridoux, Jesper S. Nielsen, Mathias B. Bertelsen, Sophie Hernot, Kurt V. Gothelf, Jørgen Kjems
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 35, Iss 3, Pp 102305- (2024)
In cancer molecular imaging, selecting binders with high specificity and affinity for biomarkers is paramount for achieving high-contrast imaging within clinical time frames. Nanobodies have emerged as potent candidates, surpassing antibodies in pre-
Externí odkaz:
https://doaj.org/article/433c78f861d246df826c2c03401e36f5
Autor:
Alexander F. Sandahl, Thuy J. D. Nguyen, Rikke A. Hansen, Martin B. Johansen, Troels Skrydstrup, Kurt V. Gothelf
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-7 (2021)
The poor bench stability of phosphoramidites is a drawback for fast automised chemical oligonucleotide synthesis. Here, the authors report a method for on-demand flow synthesis of phosphoramidites within short reaction times, in near-quantitative yie
Externí odkaz:
https://doaj.org/article/544d536eae8a41adb25c5f2ddb62b424
Autor:
Sisi Jia, Jianbang Wang, Mo Xie, Jixue Sun, Huajie Liu, Yinan Zhang, Jie Chao, Jiang Li, Lihua Wang, Jianping Lin, Kurt V. Gothelf, Chunhai Fan
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-9 (2019)
DNA origami has gained great interest for the creation of precise nanostructures. Here, the authors report on the creation of metalized DNA origami structured formed by metal condensation of DNA and nucleated metal growth on the formed DNA nanostruct
Externí odkaz:
https://doaj.org/article/7fded29116f14f4aa477fa2d18a9c707
Autor:
Mette D. E. Jepsen, Steffen M. Sparvath, Thorbjørn B. Nielsen, Ane H. Langvad, Guido Grossi, Kurt V. Gothelf, Ebbe S. Andersen
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
FRET has been used to study protein conformational changes but has never been applied to RNA aptamers. Here the authors develop a genetically encodable RNA aptamer-based FRET system on single-stranded RNA origami scaffolds, and demonstrate it can be
Externí odkaz:
https://doaj.org/article/616f157ee0b5469fa10d778ba09eeffb
Publikováno v:
Hansen, R A, Märcher, A & Gothelf, K V 2022, ' One-Step Conversion of NHS Esters to Reagents for Site-Directed Labeling of IgG Antibodies ', Bioconjugate Chemistry, vol. 33, no. 10, pp. 1811-1817 . https://doi.org/10.1021/acs.bioconjchem.2c00392
Antibody conjugates are extensively used for diagnostics and therapeutics, and as a tool for molecular biology. To prepare such conjugates N-hydroxysuccinimide (NHS) esters are most often used due to the straightforward experimental procedure and the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50d0aac560e9308f45303d5dfeb0a637
https://doi.org/10.1021/acs.bioconjchem.2c00392
https://doi.org/10.1021/acs.bioconjchem.2c00392
Publikováno v:
Kjærsgaard, N L, Hansen, R A & Gothelf, K V 2022, ' Preparation of Maleimide-Modified Oligonucleotides from the Corresponding Amines Using N-Methoxycarbonylmaleimide ', Bioconjugate Chemistry, vol. 33, no. 7, pp. 1254–1260 . https://doi.org/10.1021/acs.bioconjchem.2c00144
Oligonucleotide conjugates constitute a versatile tool for research and bioanalytical purposes. Often, such conjugates are prepared by reaction between a thiol on the protein with a maleimide-modified oligonucleotide. Unlike most other chemical handl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a7e99d88d1bee73c67865c6950700f6
https://doi.org/10.1021/acs.bioconjchem.2c00144
https://doi.org/10.1021/acs.bioconjchem.2c00144
Publikováno v:
Hansen, R A, Märcher, A, Pedersen, K N & Gothelf, K V 2023, ' Insertion of Chemical Handles into the Backbone of DNA during Solid-Phase Synthesis by Oxidative Coupling of Amines to Phosphites ', Angewandte Chemie-International Edition, vol. 62, no. 26, e202305373 . https://doi.org/10.1002/anie.202305373
Conjugation of molecules or proteins to oligonucleotides can improve their functional and therapeutic capacity. However, such modifications are often limited to the 5′ and 3′ end of oligonucleotides. Herein, we report the development of an inexpe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4072b73e9c4db78fb4e9ce9290c478f1
https://doi.org/10.1002/ange.202305373
https://doi.org/10.1002/ange.202305373
Autor:
Martin Frandsen, Kassem El‐Chami, Johan Palmfeldt, Jakob Melgaard Smidt, Maja E. T. Langballe, Alexander Sandahl, Kurt V. Gothelf
Publikováno v:
Frandsen, M, El-Chami, K, Palmfeldt, J, Smidt, J M, Langballe, M E T, Sandahl, A & Gothelf, K V 2023, ' Automated Solid-Phase Oligo(disulfide) Synthesis ', Angewandte Chemie International Edition, vol. 62, no. 23, e202303170 . https://doi.org/10.1002/anie.202303170
A method for automated solid-phase synthesis of oligo(disulfide)s was developed. It is based on a synthetic cycle comprising removal of a protecting group from a resin-bound thiol followed by treatment with monomers containing a thiosulfonate as an a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f9b91ed228369132ae5e75c14caefe7
https://doi.org/10.1002/ange.202303170
https://doi.org/10.1002/ange.202303170
Publikováno v:
Kjaersgaard, N L, Nielsen, T B & Gothelf, K V 2022, ' Chemical Conjugation to Less Targeted Proteinogenic Amino Acids ', ChemBioChem, vol. 23, no. 19, e202200245 . https://doi.org/10.1002/cbic.202200245
Protein bioconjugates are in high demand for applications in biomedicine, diagnostics, chemical biology and bionanotechnology. Proteins are large and sensitive molecules containing multiple different functional groups and in particular nucleophilic g
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9466e317a8a75cd64b0afb0e924e7843
https://pure.au.dk/portal/da/publications/chemical-conjugation-to-less-targeted-proteinogenic-amino-acids(25bc4d8a-a672-466c-a5b9-892156fdde5b).html
https://pure.au.dk/portal/da/publications/chemical-conjugation-to-less-targeted-proteinogenic-amino-acids(25bc4d8a-a672-466c-a5b9-892156fdde5b).html
Autor:
Anders Märcher, Vipin Kumar, Veronica L. Andersen, Kassem El‐Chami, Thuy J. D. Nguyen, Mads K. Skaanning, Imke Rudnik‐Jansen, Jesper S. Nielsen, Kenneth A. Howard, Jørgen Kjems, Kurt V. Gothelf
Publikováno v:
Märcher, A, Kumar, V, Andersen, V L, El-Chami, K, Nguyen, T J D, Skaanning, M K, Rudnik-Jansen, I, Nielsen, J S, Howard, K A, Kjems, J & Gothelf, K V 2022, ' Functionalized Acyclic (l)-Threoninol Nucleic Acid Four-Way Junction with High Stability In Vitro and In Vivo ', Angewandte Chemie-International Edition, vol. 61, no. 24, 202115275 . https://doi.org/10.1002/anie.202115275
Oligonucleotides are increasingly being used as a programmable connection material to assemble molecules and proteins in well-defined structures. For the application of such assemblies for in vivo diagnostics or therapeutics it is crucial that the ol
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce902a59a08814fbc4843125b4c96ca5
https://pure.au.dk/portal/da/publications/functionalized-acyclic-lthreoninol-nucleic-acid-fourway-junction-with-high-stability-in-vitro-and-in-vivo(2c7f5436-6060-4d45-9a07-53de17409047).html
https://pure.au.dk/portal/da/publications/functionalized-acyclic-lthreoninol-nucleic-acid-fourway-junction-with-high-stability-in-vitro-and-in-vivo(2c7f5436-6060-4d45-9a07-53de17409047).html