Genetic screens reveal a central role for heme metabolism in artemisinin susceptibility

Autor: Clare R. Harding, Saima M. Sidik, Boryana Petrova, Nina F. Gnädig, John Okombo, Alice L. Herneisen, Kurt E. Ward, Benedikt M. Markus, Elizabeth A. Boydston, David A. Fidock, Sebastian Lourido

Publikováno v: Nature Communications, Vol 11, Iss 1, Pp 1-18 (2020)

Artemisinin (ART) resistance poses a problem for malaria elimination. Here, the authors perform genome-wide CRISPR screens in Toxoplasma gondii and identify that the putative transporter Tmem14c and mitochondrial heme metabolism, through mitochondria

Externí odkaz: https://doaj.org/article/b8df34776e6545b698c1d120fc0d277b

Integrative Genetic Manipulation of Plasmodium cynomolgi Reveals Multidrug Resistance-1 Y976F Associated With Increased In Vitro Susceptibility to Mefloquine

Autor: Kurt E Ward, Peter Christensen, Annie Racklyeft, Satish K Dhingra, Adeline C Y Chua, Caroline Remmert, Rossarin Suwanarusk, Jessica Matheson, Michael J Blackman, Osamu Kaneko, Dennis E Kyle, Marcus C S Lee, Robert W Moon, Georges Snounou, Laurent Rénia, David A Fidock, Bruce Russell, Pablo Bifani

Publikováno v: The Journal of Infectious Diseases. 227:1121-1126

The lack of a long-term in vitro culture method has severely restricted the study of Plasmodium vivax, in part because it limits genetic manipulation and reverse genetics. We used the recently optimized Plasmodium cynomolgi Berok in vitro culture mod

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::36e238afd3c39b602fa01c6f8d778c32
https://doi.org/10.1093/infdis/jiac469

Plasmodium falciparum K13 mutations in Africa and Asia impact artemisinin resistance and parasite fitness

Autor: Sachel Mok, Adama Gansané, Rithea Leang, Clark H. Cunningham, Romaric Nzoumbou-Boko, David A. Fidock, Aline Uwimana, Ioanna Deni, Nina F. Gnädig, Barbara H. Stokes, Marian Warsame, Kelly Rubiano, Leila S. Ross, Olimatou Kolley, Kurt E. Ward, Issa M. Souleymane, Eric Legrand, Frédéric Ariey, Philip J. Rosenthal, Josefine Striepen, Samuel J. Smith, Abdunoor M. Kabanywanyi, Judith Straimer, Jade Bath, Kyra A Schindler, Didier Menard, Mathieu Ndounga, Tim J. Anderson, François Nosten, Claudette Ndayikunda, Tomas Yeo, Satish K. Dhingra

Publikováno v: eLife, Vol 10 (2021)
eLife
eLife, eLife Sciences Publication, 2021, 10, pp.e66277. ⟨10.7554/eLife.66277⟩
eLife, 2021, 10, pp.e66277. ⟨10.7554/eLife.66277⟩

The emergence of mutant K13-mediated artemisinin (ART) resistance in Plasmodium falciparum malaria parasites has led to widespread treatment failures across Southeast Asia. In Africa, K13-propeller genotyping confirms the emergence of the R561H mutat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a9d4652f8546d77e0464272dc94dc4b6
https://ora.ox.ac.uk/objects/uuid:24d02c06-c99a-469c-92f8-1d3ccb074394

Author response: Plasmodium falciparum K13 mutations in Africa and Asia impact artemisinin resistance and parasite fitness

Autor: Barbara H Stokes, Satish K Dhingra, Kelly Rubiano, Sachel Mok, Judith Straimer, Nina F Gnädig, Ioanna Deni, Kyra A Schindler, Jade R Bath, Kurt E Ward, Josefine Striepen, Tomas Yeo, Leila S Ross, Eric Legrand, Frédéric Ariey, Clark H Cunningham, Issa M Souleymane, Adama Gansané, Romaric Nzoumbou-Boko, Claudette Ndayikunda, Abdunoor M Kabanywanyi, Aline Uwimana, Samuel J Smith, Olimatou Kolley, Mathieu Ndounga, Marian Warsame, Rithea Leang, François Nosten, Timothy JC Anderson, Philip J Rosenthal, Didier Ménard, David A Fidock

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6f082a2bee69442e99061c71310505b8
https://doi.org/10.7554/elife.66277.sa2

P. falciparum K13 mutations present varying degrees of artemisinin resistance and reduced fitness in African parasites

Autor: Tim J. Anderson, Eric Legrand, Samuel J. Smith, Marian Warsame, Kelly Rubiano, Claudette Ndayikunda, Romaric Nzoumbou-Boko, Philip J. Rosenthal, Ioanna Deni, Barbara H. Stokes, Tomas Yeo, Josefine Striepen, Kurt E. Ward, François Nosten, Satish K. Dhingra, Sachel Mok, Leila S. Ross, Aline Uwimana, Clark H. Cunningham, Rithea Leang, Mathieu Ndounga, Issa M. Souleymane, Frédéric Ariey, David A. Fidock, Abdunoor M. Kabanywanyi, Jade Bath, Judith Straimer, Didier Menard, Nina F. Gnädig, Adama Gansané, Olimatou Kolley

The emergence of artemisinin (ART) resistance in Plasmodium falciparum parasites, driven by K13 mutations, has led to widespread antimalarial treatment failure in Southeast Asia. In Africa, our genotyping of 3,299 isolates confirms the emergence of t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::7fa371c484ab5b5a9da57d152378813a
https://doi.org/10.1101/2021.01.27.428390