Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection

Autor: Katie A. Mitzelfelt, Chris McDermott-Roe, Michael N. Grzybowski, Maribel Marquez, Chieh-Ti Kuo, Michael Riedel, Shuping Lai, Melinda J. Choi, Kurt D. Kolander, Daniel Helbling, David P. Dimmock, Michele A. Battle, Chuanchau J. Jou, Martin Tristani-Firouzi, James W. Verbsky, Ivor J. Benjamin, Aron M. Geurts

Publikováno v: Stem Cell Reports, Vol 8, Iss 3, Pp 491-499 (2017)

Genome editing in induced pluripotent stem cells is currently hampered by the laborious and expensive nature of identifying homology-directed repair (HDR)-modified cells. We present an approach where isolation of cells bearing a selectable, HDR-media

Externí odkaz: https://doaj.org/article/83b03a2018cb4c5d8fd10a28c1724404

The Human 343delT HSPB5 Chaperone Associated with Early-onset Skeletal Myopathy Causes Defects in Protein Solubility

Autor: J. Hudson, Ivor J. Benjamin, Qiang Dai, Justin L. P. Benesch, Elisabeth S. Christians, Rita Barresi, Alex C. Minella, Michael Grzybowski, Kate Bushby, Aron M. Geurts, Pattraranee Limphong, Elizabeth Wraige, Michael Riedel, Heinz Jungbluth, Mai T. Thao, Frances D.L. Kondrat, Shuping Lai, Huali Zhang, Melinda J. Choi, Wai-Meng Kwok, Qiang Lui, Graydon Taylor, Kurt D. Kolander, Katie A. Mitzelfelt

Publikováno v: Mitzelfelt, K A, Limphong, P, Choi, M J, Kondrat, F D L, Lai, S, Kolander, K D, Kwok, W-M, Dai, Q, Grzybowski, M N, Zhang, H, Taylor, G M, Lui, Q, Thao, M T, Hudson, J A, Barresi, R, Bushby, K, Jungbluth, H, Wraige, E, Geurts, A M, Benesch, J L P, Riedel, M, Christians, E S, Minella, A C & Benjamin, I J 2016, ' Human 343delT HSPB5 Chaperone associated with Early-onset Skeletal Myopathy causes Defects in Protein Solubility ', Journal of Biological Chemistry . https://doi.org/10.1074/jbc.M116.730481

Mutations of HSPB5 (also known as CRYAB or αB-crystallin), a bona fide heat shock protein and molecular chaperone encoded by the HSPB5 (crystallin, alpha B) gene, are linked to various multisystem disorders featuring variable combinations of catarac

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b85b51dad93daa504c4b792be2ca5fe7
https://doi.org/10.1074/jbc.m116.730481