Výsledky vyhledávání - "Kuo-Liang Hsi"

An Alternative Method for the Isolation and Identification of C-Terminal Peptide of Protein

Autor: Kuo-Liang Hsi, Lydia M. Nuwaysir, David R. Dupont

Publikováno v: Protein & Peptide Letters. 6:195-201

Abstract: A chemical approach for selectively isolating the C-terminal peptide from a protein is described. The protein to be analyzed is derivatized with glycinamide to protect carboxyl groups at both C-terminus and Asp/Glu side chains and subsequen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::2aa99cbf63ddf8c817d9cbe55b0b079e
https://doi.org/10.2174/092986650604221115150348

Zobrazit plný text záznamu

Microscale isolation of blocked n-terminal peptides and sequence analysis by mass spectrometry

Autor: Kuo-Liang Hsi, Lydia Nuwaysir, Dave Dupont, Pau-Miau Yuan

Publikováno v: Protein & Peptide Letters. 5:243-250

Abstract: An improved procedure for selectively isolating a blocked N-terminal is described to work with low microgram amounts of protein. Additionally, the scope of this method is expanded to include selective isolation of free and blocked N-termini

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e0101e462bbc5e4a69281b876bdb1748
https://doi.org/10.2174/092986650505221111091131

Zobrazit plný text záznamu

Visualization of Proteins by Modification of Lysines, Cysteines, and Phosphorylated Serines Facilitates Sample Preparation for Microsequencing

Autor: David R. Dupont, Pau-Miau Yuan, Steven A. O'Neill, Kuo-Liang Hsi

Publikováno v: Analytical Biochemistry. 258:38-47

A procedure for visualization and sensitive detection of protein during sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and subsequent sample preparation for sequence analysis is described. This procedure utilizes either fluo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce345b7eaa3e2132c6fba23cf8d374c8
https://doi.org/10.1006/abio.1998.2582

Zobrazit plný text záznamu

Development of a capillary lc blotting systems for low and sub-picomole sequencing sample preparation

Autor: Kuo-Liang Hsi, Michael L. Kochersperger, William E. Werner, Chris H. Grimley, Lynn R. Zieske, Pau-Miau Yuan

Publikováno v: Protein & Peptide Letters. 4:1-8

Abstract: A capillary liquid chromatography (cLC)/microblotting system, 173A MicroBlotter, has recently been developed [1,2]. This newly designed system consists of a capillary LC for sample separation and an on-line microblotter for direct collectio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::1de30be3b01e30a812b638f60c10c1ab
https://doi.org/10.2174/092986650401221012133013

Zobrazit plný text záznamu

Structural determination of the essential serine and glycosylation sites of carboxypeptidase P

Autor: Pau-Miau Yuan, Kuo-Liang Hsi, Lynn R. Zieske, Ling Chen

Publikováno v: Archives of Biochemistry and Biophysics. 295:76-83

Through a series of kinetic studies involving the inactivation effects of diisopropylfluorophosphate, an affinity label that modifies the active site serine residue involved in the mechanism of action, it has been firmly established that carboxypepti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa71c3c3e0130d0a3fae1bddf4be45e1
https://doi.org/10.1016/0003-9861(92)90490-n

Zobrazit plný text záznamu

Human DNA polymerase α catalytic polypeptide binds ConA and RCA and contains a specific labile site in the N-terminus

Autor: Teresa S.-F. Wang, William C. Copeland, Kuo-Liang Hsi

Publikováno v: Nucleic Acids Research. 18:6231-6237

The catalytic polypeptide of DNA polymerase alpha is often observed in vitro as a family of phosphopolypeptides predominantly of 180 and 165 kDa derived from a single primary structure. The estimated Mr of this polypeptide deduced from the full-lengt

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0def0adb385658830c064f9ed829623e
https://doi.org/10.1093/nar/18.21.6231

Zobrazit plný text záznamu

A strategy of obtain internal sequence information from blotted proteins after initial N-terminal sequencing

Autor: Steven A. O'Neill, William E. Werner, Kent Yamada, Pau-Miau Yuan, Lynn R. Zieske, Kuo-Liang Hsi, Michael L. Kochersperger, Chris Grimley

Publisher Summary To generate internal peptide fragments for the identification of sequences from N-terminally blocked proteins, or for the maximization of sequence, information from larger proteins require purification of additional protein sample.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::85fcfc475472df72ca9317c41cf9bbd6
https://doi.org/10.1016/s1080-8914(97)80012-9

Zobrazit plný text záznamu

A Practical Approach for Isolation and Characterization of Glycosylation Sites of Glycoproteins Bearing N- and/or O-Linked Carbohydrate Chains

Autor: Ling Chen, Kuo-Liang Hsi, Pau-Miau Yuan

Publisher Summary This chapter presents a practical approach for isolation and characterization of glycosylation sites of glycoproteins bearing N- and/or O-linked carbohydrate chains. Two glycoproteins were chosen as model glycoproteins for this stud

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::66cc100248a3094dc2b65faee6507cc9
https://doi.org/10.1016/b978-0-12-058757-5.50021-4

Zobrazit plný text záznamu

A Unified Approach to Glycoprotein Primary Structure Analysis

Autor: Pau-Miau Yuan, Ling Chen, David H. Hawke, Lynn R. Zieske, Kuo-Liang Hsi

Publisher Summary This chapter outlines the general strategy for the analysis of glycoproteins, utilizing lectin staining, available peptide separation and sequencing techniques, a simple micro-batch affinity method, PDMS, and PMP labeling of carbohy

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6e87389da66a2c9b3ac36678335f8a4f
https://doi.org/10.1016/b978-0-12-058756-8.50037-7

Zobrazit plný text záznamu

Vyhledávací nástroje:

Upřesnit hledání