Zobrazeno 1 - 10
of 206
pro vyhledávání: '"Kunio Ohmiya"'
Autor:
Truong Thi Hoa, Ajay Singh, Kenji Iwakiri, Chika Aoyagi, Ken Tokuyasu, Kunio Ohmiya, Kiyoshi Hayashi
Publikováno v:
Brazilian Archives of Biology and Technology, Vol 42, Iss 3, Pp 303-306 (1999)
Based on homology search in the amino acid sequences, a chimeric ß-glucosidase with enzyme activity has been prepared by replacing C-terminal region of Thr509-Arg646 from Cellvibrio gilvus with N-terminal region of Arg160-Gly283 from Ruminococcus al
Externí odkaz:
https://doaj.org/article/c3c723825c2b44d590d54476b9c4dd1c
Publikováno v:
Bioscience, biotechnology, and biochemistry. 57(2)
The nucleotides of the β-xylosidase (xylA) gene from Clostridium stercorarium were sequenced. A single open reading frame of 473 codons specifying the subunit (MW 53,340) of xylosidase was identified. The N-terminal amino acid sequence and molecular
Autor:
Kunio Ohmiya, Hayato Umekawa, Tetsuya Kimura, Hideo Miyake, Kazuo Sakka, Guangshan Zhao, Huazhong Li
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 70:1127-1133
Clostridium paraputrificum M-21 beta-N-acetylglucosaminidase 3A (Nag3A) is an enzyme classified in family 3 of the glycoside hydrolases. To identify catalytic residues of this enzyme, mutations were introduced into highly conserved Glu and Asp residu
Publikováno v:
World Journal of Microbiology and Biotechnology. 22:331-335
A DNA fragment encoding for 598 amino acids of chitinase protein from Bacillus circulans No. 4.1 was subcloned into pQE-30 expression vector and transformed into Escherichia coli M15 (pREP4). The molecular weight of the expressed protein was approxim
Publikováno v:
Biotechnology and Bioprocess Engineering. 10:482-493
Biomass is originally photosynthesized from inorgainic compounds such as CO2, minerals, water and solar energy. Recent studies have shown that anaerobic bacteria have the ability to convert recalcitrant biomass such as cellulosic or chitinoic materia
Autor:
Rie Mizuno, Masami Shimizu, Tetsuro Jinbo, Tetsuya Kimura, Takayoshi Koyama, Keiji Tomita, Toshiro Ono, Kazuo Sakka, Tetsu Kawazu, Norihiro Mitsukawa, Kunio Ohmiya
Publikováno v:
Journal of Bioscience and Bioengineering. 99:38-42
The PHT1 promoter::GUS fusion gene was constructed and introduced into Arabidopsis and rice by Agrobacterium-mediated transformation. Strong beta-glucuronidase (GUS) activity was detected in roots and showed phosphate starvation induction both in Ara
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 69:160-165
Clostridium thermocellum xylanase Xyn10C (formerly XynC) is a modular enzyme, comprising a family-22 carbohydrate-binding module (CBM), a family-10 catalytic module of the glycoside hydrolases, and a dockerin module responsible for cellulosome assemb
Autor:
Shuichi Karita, Akane Soda, Tetsuya Kimura, Kunio Ohmiya, Minoru Inagaki, Sadanari Jindou, Tsutomu Kajino, Kazuo Sakka, Pierre Béguin, J.H. David Wu
Publikováno v:
Journal of Biological Chemistry. 279:9867-9874
The cellulosome components are assembled into the cellulosome complex by the interaction between one of the repeated cohesin domains of a scaffolding protein and the dockerin domain of an enzyme component. We prepared five recombinant cohesin polypep
Publikováno v:
Applied Microbiology and Biotechnology. 62:374-379
The gene encoding the catalytic domain of thermostable xylanase from Clostridium thermocellum F1 was expressed in rice plants under the control of a constitutive promoter. The gene encoding Xylanase A was modified to encode the catalytic domain of fa