Zobrazeno 1 - 10
of 25
pro vyhledávání: '"Kunal B Roy"'
Publikováno v:
PLoS ONE, Vol 8, Iss 5, p e65010 (2013)
We have designed a dodecamer d-AGATCTAGATCT (RY12) with alternate oligopurines and oligopyrimidines tracts and its homologous 28 bp hairpin oligomer (RY28) that forms a triple helix only in the presence of a pentapeptide REWER. An intermolecular trip
Externí odkaz:
https://doaj.org/article/33483ae2bbf0441989a910a27c77935c
Publikováno v:
Biochemistry. 37:15542-15547
The contribution of ATP and other nucleotides to the stabilization of non-native structures has been described for some proteins. We report here the effect of GTP, ATP, and their nonhydrolyzable analogues on the denaturation and renaturation of the e
Publikováno v:
PLoS ONE
PLoS ONE, Vol 8, Iss 5, p e65010 (2013)
PLoS ONE, Vol 8, Iss 5, p e65010 (2013)
We have designed a dodecamer d-AGATCTAGATCT (RY12) with alternate oligopurines and oligopyrimidines tracts and its homologous 28 bp hairpin oligomer (RY28) that forms a triple helix only in the presence of a pentapeptide REWER. An intermolecular trip
Publikováno v:
Journal of Biomolecular Structure and Dynamics. 11:1041-1048
The hairpin-duplex equilibria of the dodecamer d-AAGCTTAAGCTT and interaction of the duplex form with a pentapeptide, KGWGK, has been studied. UV thermal transitions are monophasic at low salt but biphasic at higher salt concentrations. At 10(-5) M o
Autor:
Alka Gupta, Himangshu S. Bose, Virander S. Chauhan, Kunal B. Roy, Moganty R. Rajeswari, Shrikant Kukreti
Publikováno v:
Biochemistry. 31:6237-6241
The interactions of three tryptophan-containing peptides, KWK, KGWK tert-butyl ester, and KGWGK, with two self-complementary dodecamers of the same base composition but different sequence were studied by UV, CD, and fluorescence spectroscopy. The oli
Autor:
Arun K. Shukla, Kunal B. Roy
Publikováno v:
Biological chemistry. 387(3)
We have recently reported that a GC-rich palindromic repeat sequence presumably adopts a stable fold-back tetraplex DNA structure under supercoiling. To establish the biological significance of this structure, we inserted this sequence between two di
Autor:
Arun K. Shukla, Kunal B. Roy
Publikováno v:
Journal of biochemistry. 139(1)
A synthetic deoxyoligonucleotide containing five palindromic repeats of GGATCC self assembles to form a parallel four-stranded structure held together by G-tetrads that shows slower mobility than duplex DNA. This structure is hypersensitive to S1 nuc
Publikováno v:
Japanese journal of infectious diseases. 55(5)
A Plasmodium falciparum malaria blood stage antigen was isolated from in vitro parasite culture supernatant. The chemical composition of the antigen was studied by high-performance thin-layer chromatography, thin-layer chromatography, gas-liquid chro
Autor:
Kunal B. Roy, Asha S. Acharya
Publikováno v:
Biochemical and biophysical research communications. 287(1)
Three specific mutants, C54I, C54W, and a double-mutant C54D:C64R of restriction endonuclease BamHI, were generated and studied to investigate the role, if any, of the 54th and 64th cysteine residues in the catalysis of BamHI. The mutation was achiev
Autor:
Sonia Advani, Kunal B. Roy
Publikováno v:
Biochemical and biophysical research communications. 279(1)
Biochemical properties of Type II restriction enzyme Ban I were characterized. Kinetic parameters were evaluated and an enhancement of rate was observed when the recognition site was located in a more central position in the substrate, suggesting tha