Development and validation of a FACS-based lipoprotein localization screen in the Lyme disease spirochete Borrelia burgdorferi

Autor: Slusser Joyce G, Schulze Ryan J, Kumru Ozan S, Zückert Wolfram R

Publikováno v: BMC Microbiology, Vol 10, Iss 1, p 277 (2010)

Abstract Background In our previous studies on lipoprotein secretion in the Lyme disease spirochete Borrelia burgdorferi, we used monomeric red fluorescent protein 1 (mRFP1) fused to specifically mutated outer surface protein A (OspA) N-terminal lipo

Externí odkaz: https://doaj.org/article/8935dd34401345f79bd2f98be4309b93

Engineered SARS-CoV-2 receptor binding domain improves immunogenicity in mice and elicits protective immunity in hamsters.

Autor: Dalvie NC; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA., Rodriguez-Aponte SA; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA., Hartwell BL; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.; Ragon Institute of MGH, MIT, Harvard, Cambridge, MA 02139, USA., Tostanoski LH; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA., Biedermann AM; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA., Crowell LE; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA., Kaur K; Department of Pharmaceutical Chemistry, Vaccine Analytics and Formulation Center, University of Kansas, Lawrence, Kansas, 66047, United States., Kumru O; Department of Pharmaceutical Chemistry, Vaccine Analytics and Formulation Center, University of Kansas, Lawrence, Kansas, 66047, United States., Carter L; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Yu J; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA., Chang A; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.; Harvard Medical School, Boston, MA 02115, USA., McMahan K; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA., Courant T; Vaccine Formulation Institute, 1228 Plan-Les-Ouates, Geneva, Switzerland., Lebas C; Vaccine Formulation Institute, 1228 Plan-Les-Ouates, Geneva, Switzerland., Lemnios AA; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA., Rodrigues KA; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.; Ragon Institute of MGH, MIT, Harvard, Cambridge, MA 02139, USA.; Harvard-MIT Health Sciences and Technology, Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Silva M; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA., Johnston RS; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA., Naranjo CA; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA., Tracey MK; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA., Brady JR; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA., Whittaker CA; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA., Yun D; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA., Kar S; Bioqual, Inc., Rockville, MD 20850, USA., Porto M; Bioqual, Inc., Rockville, MD 20850, USA., Lok M; Bioqual, Inc., Rockville, MD 20850, USA., Andersen H; Bioqual, Inc., Rockville, MD 20850, USA., Lewis MG; Bioqual, Inc., Rockville, MD 20850, USA., Love KR; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA., Camp DL; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA., Silverman JM; Bill & Melinda Gates Medical Research Institute, Cambridge, MA 02139, USA., Kleanthous H; Bill&Melinda Gates Foundation, Seattle, WA 98109, USA., Joshi SB; Department of Pharmaceutical Chemistry, Vaccine Analytics and Formulation Center, University of Kansas, Lawrence, Kansas, 66047, United States., Volkin DB; Department of Pharmaceutical Chemistry, Vaccine Analytics and Formulation Center, University of Kansas, Lawrence, Kansas, 66047, United States., Dubois PM; Vaccine Formulation Institute, 1228 Plan-Les-Ouates, Geneva, Switzerland., Collin N; Vaccine Formulation Institute, 1228 Plan-Les-Ouates, Geneva, Switzerland., King NP; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Barouch DH; Ragon Institute of MGH, MIT, Harvard, Cambridge, MA 02139, USA.; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.; Harvard Medical School, Boston, MA 02115, USA.; Massachusetts Consortium on Pathogen Readiness, Boston, MA 02115, USA., Irvine DJ; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.; Ragon Institute of MGH, MIT, Harvard, Cambridge, MA 02139, USA.; Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA., Love JC; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2021 Mar 04. Date of Electronic Publication: 2021 Mar 04.