Protein engineering of a ubiquitin-variant inhibitor of APC/C identifies a cryptic K48 ubiquitin chain binding site.

Autor: Watson ER; Department of Structural Biology, St. Jude Children's Research Hospital, Memphis, TN 38105.; Department of Molecular Machines and Signaling, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany., Grace CRR; Department of Structural Biology, St. Jude Children's Research Hospital, Memphis, TN 38105., Zhang W; Donnelly Centre for Cellular and Biomolecular Research, Banting and Best Department of Medical Research, University of Toronto, Toronto, ON, Canada M5S3E1.; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada M5S3E1., Miller DJ; Department of Structural Biology, St. Jude Children's Research Hospital, Memphis, TN 38105., Davidson IF; Research Institute of Molecular Pathology, Vienna BioCenter, 1030 Vienna, Austria., Prabu JR; Department of Molecular Machines and Signaling, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany., Yu S; Department of Structural Biology, St. Jude Children's Research Hospital, Memphis, TN 38105., Bolhuis DL; Department of Pharmacology and Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599., Kulko ET; Department of Pharmacology and Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599., Vollrath R; Department of Molecular Machines and Signaling, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany., Haselbach D; Research Institute of Molecular Pathology, Vienna BioCenter, 1030 Vienna, Austria.; Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany., Stark H; Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany., Peters JM; Research Institute of Molecular Pathology, Vienna BioCenter, 1030 Vienna, Austria.; Medical University of Vienna, 1090 Vienna, Austria., Brown NG; Department of Structural Biology, St. Jude Children's Research Hospital, Memphis, TN 38105.; Department of Pharmacology and Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599., Sidhu SS; Donnelly Centre for Cellular and Biomolecular Research, Banting and Best Department of Medical Research, University of Toronto, Toronto, ON, Canada M5S3E1; sachdev.sidhu@utoronto.ca schulman@biochem.mpg.de., Schulman BA; Department of Structural Biology, St. Jude Children's Research Hospital, Memphis, TN 38105; sachdev.sidhu@utoronto.ca schulman@biochem.mpg.de.; Department of Molecular Machines and Signaling, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany.

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2019 Aug 27; Vol. 116 (35), pp. 17280-17289. Date of Electronic Publication: 2019 Jul 26.