Characterizing the portability of phage-encoded homologous recombination proteins.

Autor: Filsinger GT; Department of Systems Biology, Harvard Medical School, Boston, MA, USA. gabrieltfilsinger@gmail.com.; Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA, USA. gabrieltfilsinger@gmail.com., Wannier TM; Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA, USA.; Department of Genetics, Harvard Medical School, Boston, MA, USA., Pedersen FB; Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark., Lutz ID; Institute for Protein Design, University of Washington, Seattle, WA, USA.; Department of Bioengineering, University of Washington, Seattle, WA, USA., Zhang J; Department of Mathematics, Massachusetts Institute of Technology, Cambridge, MA, USA., Stork DA; Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA, USA.; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, USA., Debnath A; Department of Genetics, Harvard Medical School, Boston, MA, USA.; Tenza Inc., Cambridge, MA, USA., Gozzi K; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Kuchwara H; Department of Genetics, Harvard Medical School, Boston, MA, USA., Volf V; Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA, USA.; John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA., Wang S; Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA, USA.; Department of Genetics, Harvard Medical School, Boston, MA, USA., Rios X; Department of Genetics, Harvard Medical School, Boston, MA, USA., Gregg CJ; Department of Genetics, Harvard Medical School, Boston, MA, USA., Lajoie MJ; Department of Genetics, Harvard Medical School, Boston, MA, USA., Shipman SL; Gladstone Institutes, San Francisco, CA, USA.; Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA, USA., Aach J; Department of Genetics, Harvard Medical School, Boston, MA, USA., Laub MT; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Church GM; Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA, USA. gchurch@genetics.med.harvard.edu.; Department of Genetics, Harvard Medical School, Boston, MA, USA. gchurch@genetics.med.harvard.edu.

Publikováno v: Nature chemical biology [Nat Chem Biol] 2021 Apr; Vol. 17 (4), pp. 394-402. Date of Electronic Publication: 2021 Jan 18.

Editing of Phage Genomes—Recombineering-assisted SpCas9 Modification of Model Coliphages T7, T5, and T3.

Autor: Isaev, A., Andriianov, A., Znobishcheva, E., Zorin, E., Morozova, N., Severinov, K.

Publikováno v: Molecular Biology; Dec2022, Vol. 56 Issue 6, p801-815, 15p

SURE editing: combining oligo-recombineering and programmable insertion/deletion of selection markers to efficiently edit the Mycoplasma pneumoniae genome.

Autor: Piñero-Lambea, Carlos, Garcia-Ramallo, Eva, Miravet-Verde, Samuel, Burgos, Raul, Scarpa, Margherita, Serrano, Luis, Lluch-Senar, Maria

Publikováno v: Nucleic Acids Research; 12/9/2022, Vol. 50 Issue 22, pe127-e127, 1p

Improved dsDNA recombineering enables versatile multiplex genome engineering of kilobase-scale sequences in diverse bacteria.

Autor: Wang, Xue, Zheng, Wentao, Zhou, Haibo, Tu, Qiang, Tang, Ya-Jie, Stewart, A Francis, Zhang, Youming, Bian, Xiaoying

Publikováno v: Nucleic Acids Research; 2/22/2022, Vol. 50 Issue 3, pe15-e15, 1p