Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Kristy L. Kounovsky-Shafer"'
Publikováno v:
ELECTROPHORESIS. 44:744-751
Autor:
Cody Masters, Haishi Cao, Haley A. Houtwed, Aatiya Ahmad, Kristy L. Kounovsky-Shafer, Melissa M. Davison, Meng Xie
Publikováno v:
J Fluoresc
NCy3, a derivative of Cyanine 3 with a nitro substituent, showed a high reactivity to bisulfite in aqueous media, instantly leading to ratiometric change of absorption spectra and significant fluorescence quenching. Applied in the microfluidic channe
Autor:
Keegan McGill, April Maschmann, Matthew Moore, Cody Masters, Drew Thompson, Kristy L. Kounovsky-Shafer, Jocelyn Dolphin
Publikováno v:
ELECTROPHORESIS. 40:810-816
To understand structural variation for personal genomics, an extensive ensemble of large DNA molecules will be required to span large structural variations. Nanocoding, a whole-genome analysis platform, can analyze large DNA molecules for the constru
Autor:
David C. Schwartz, Theo Odijk, G. Tsvid, Michael Place, Juan J. de Pablo, Juan P. Hernández-Ortiz, Prabu Ravindran, Kristy L. Kounovsky-Shafer, Shiguo Zhou, Konstantinos Potamousis, Kyubong Jo
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America
Significance Repeated sequences make up approximately two-thirds of the human genome, which become fully accountable when very large DNA molecules are analyzed. Long, single DNA molecules are problematic using common experimental techniques and fluid
Publikováno v:
Nucleosides, Nucleotides & Nucleic Acids. 36:406-417
Optical mapping, a single DNA molecule genome analysis platform that can determine methylation profiles, uses fluorescently labeled DNA molecules that are elongated on the surface and digested with a restriction enzyme to produce a barcode of that mo
Autor:
Drew Thompson, April Maschmann, Kristy L. Kounovsky-Shafer, Joshua Lallman, Melissa M. Davison, Cody Masters
Publikováno v:
Journal of Visualized Experiments.
Visualization of DNA for fluorescence microscopy utilizes a variety of dyes such as cyanine dyes. These dyes are utilized due to their high affinity and sensitivity for DNA. In order to determine if the DNA molecules are full length after the complet
Publikováno v:
Electrophoresis. 39(5-6)
Nanocoding, a genome analysis platform, relies on very low ionic strength conditions to elongate DNA molecules up to 1.06 (fully stretched DNA = 1). Understanding how electroosmotic and electrophoretic forces vary, as ionic strength decreases, will e
Publikováno v:
Microfluidics and Nanofluidics. 20
Recent application of microfluidic and nanofluidic approaches to genomics has enabled rapid analysis of whole human and other large genomes. Here, we present a review of optical mapping and nanocoding, two single-molecule whole-genome analysis system
Publikováno v:
Biophysical Journal. 110(3)
Nanocoding, a genome analysis platform, relies on very low ionic strength conditions to elongate DNA molecules up to 1.06 (fully stretched DNA molecule = 1)1, which is the largest stretch reported in the literature. Understanding how electroosmotic a
Autor:
Theo Odijk, David C. Schwartz, Kristy L. Kounovsky-Shafer, Juan J. de Pablo, Juan P. Hernández-Ortiz, Kyubong Jo
The analysis of very large DNA molecules intrinsically supports long-range, phased sequence information, but requires new approaches for their effective presentation as part of any genome analysis platform. Using a multi-pronged approach that marshal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5db59fca4062d96faf937605b6ab2bc
https://europepmc.org/articles/PMC3964590/
https://europepmc.org/articles/PMC3964590/