Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Kristopher Zimmerman"'
Autor:
Emily A. Torio, Valerie T. Ressler, Virginia A. Kincaid, Robin Hurst, Mary P. Hall, Lance P. Encell, Kristopher Zimmerman, Stuart K. Forsyth, William M. Rehrauer, Molly A. Accola, Chia-Chang Hsu, Thomas Machleidt, Melanie L. Dart
Publikováno v:
Frontiers in Microbiology, Vol 13 (2022)
Point-of-care tests are highly valuable in providing fast results for medical decisions for greater flexibility in patient care. Many diagnostic tests, such as ELISAs, that are commonly used within clinical laboratory settings require trained technic
Externí odkaz:
https://doaj.org/article/9343fd9d3e9b4dcaa378e95d0d22ecaf
Autor:
Michael P. Killoran, Sergiy Levin, Michelle E. Boursier, Kristopher Zimmerman, Robin Hurst, Mary P. Hall, Thomas Machleidt, Thomas A. Kirkland, Rachel Friedman Ohana
Publikováno v:
Molecules, Vol 26, Iss 10, p 2857 (2021)
Gaining insight into the pharmacology of ligand engagement with G-protein coupled receptors (GPCRs) under biologically relevant conditions is vital to both drug discovery and basic research. NanoLuc-based bioluminescence resonance energy transfer (Na
Externí odkaz:
https://doaj.org/article/6603ab2a2c3e4fc183fbdeba047372cc
Autor:
Rachel Friedman Ohana, Keith V. Wood, Robin Hurst, Kristopher Zimmerman, Michael M. Rosenblatt, Sergiy Levin, Thomas Machleidt, Thomas A. Kirkland
Publikováno v:
ACS Chemical Biology. 16:404-413
Identification of physiologically relevant targets for lead compounds emerging from drug discovery screens is often the rate-limiting step toward understanding their mechanism of action and potential for undesired off-target effects. To this end, we
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::829a63b86a7cd4cbc4d592003531612a
https://doi.org/10.1021/acschembio.0c00987
https://doi.org/10.1021/acschembio.0c00987
Autor:
Matthew B. Robers, Ce Shi, Keith V. Wood, Kristopher Zimmerman, Marie K. Schwinn, James Robert Hartnett, Melanie Dart, Thomas Machleidt, Michael P Killoran, Emily Jost, Jennifer Wilkinson, Thomas A. Kirkland
Publikováno v:
ACS medicinal chemistry letters. 9(6)
Protein thermal shift assays (TSAs) provide a means for characterizing target engagement through ligand-induced thermal stabilization. Although these assays are widely utilized for screening libraries and validating hits in drug discovery programs, t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cbc6ac143d89546b3bfee2252e1b9461
https://pubmed.ncbi.nlm.nih.gov/29937980
https://pubmed.ncbi.nlm.nih.gov/29937980
Autor:
Carina Glas, Kristin G. Huwiler, Thomas A. Kirkland, Kristopher Zimmerman, James D Vasta, Cesear Corona, Stefan Knapp, Chad Zimprich, Matthew B. Robers, Ke Ding, Paul Otto, Frank Fan, Susanne Müller, James Robert Hartnett, Thomas Machleidt, Benedict-Tilman Berger, Jennifer Wilkinson, Keith V. Wood, David H. Drewry, Poncho Meisenheimer, Kilian Huber, Carrow I. Wells, Mei Cong, Thomas Hanke, Morgan R. Ingold, Timothy M. Willson, Rachel Friedman Ohana, Michael R. Slater
Publikováno v:
Cell Chemical Biology
Summary For kinase inhibitors, intracellular target selectivity is fundamental to pharmacological mechanism. Although a number of acellular techniques have been developed to measure kinase binding or enzymatic inhibition, such approaches can fail to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fcc8d115cc6a4e1bbbbadcb2b8ee032a
https://doi.org/10.1016/j.chembiol.2017.10.010
https://doi.org/10.1016/j.chembiol.2017.10.010
Autor:
Brock Binkowski, Mary P. Hall, Monika G. Wood, Kristopher Zimmerman, Paul Otto, Keith V. Wood, Gediminas Vidugiris, Dieter Klaubert, Hélène A Benink, Matthew B. Robers, James Unch, Lance P. Encell, Michael P. Valley, Frank Fan, Poncho Meisenheimer, Braeden L. Butler, Thomas Machleidt, Christopher T. Eggers, Michael R. Slater
Publikováno v:
ACS Chemical Biology
Bioluminescence methodologies have been extraordinarily useful due to their high sensitivity, broad dynamic range, and operational simplicity. These capabilities have been realized largely through incremental adaptations of native enzymes and substra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b14760e5049c02c7d69b1883d21b6ca
https://doi.org/10.1021/cb3002478
https://doi.org/10.1021/cb3002478
Autor:
Mei Cong, Matthew B. Robers, Monika G. Wood, Kevin Kupcho, Keith V. Wood, James Robert Hartnett, Sergiy Levin, Thomas A. Kirkland, Danette L. Daniels, Carolyn C. Woodroofe, Andrew L. Niles, Melanie Dart, Kristopher Zimmerman, Thomas Machleidt, Chad Zimprich, Rachel Friedman Ohana, Yi-Qiang Cheng, Michael R. Slater
Publikováno v:
Nature Communications
The therapeutic action of drugs is predicated on their physical engagement with cellular targets. Here we describe a broadly applicable method using bioluminescence resonance energy transfer (BRET) to reveal the binding characteristics of a drug with
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16129acbaa5af847dd39d9c7736dbc2f
http://europepmc.org/articles/PMC4686764
http://europepmc.org/articles/PMC4686764