Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Kristine Sigrist-Nelson"'
Publikováno v:
European Journal of Biochemistry. 166:453-459
The sulfoxide agent Ro 18-5364 is an extremely potent and rapid inhibitor of the gastric mucosal (H+ + K+)-ATPase with an apparent Ki of 0.1 microM at pH 6. The inhibition of both enzymatic activity and vesicular proton transport in membrane preparat
Autor:
Nathan Nelson, Kristine Sigrist-Nelson, Bat-El Notsani, Esther Eytan, Hans Sigrist, Carlos Gitler
Publikováno v:
Proceedings of the National Academy of Sciences. 74:2375-2378
The N,N'-dicyclohexylcarbodiimide-binding proteolipid from lettuce chloroplast membranes has been purified by a novel, rapid technique involving I-butanol extraction and ether precipitation. Reconstitution of this proteolipid into liposomes composed
Publikováno v:
Biochemical and Biophysical Research Communications. 74:178-184
An alternative method to the chloroform/methanol extraction of proteolipids is presented. The proteolipid fraction from sarcoplasmic reticulum membranes is isolated by a single-phase n-butanol extraction and subsequently precipitated by diethyl ether
Autor:
Kristine Sigrist-Nelson
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Biomembranes. 394:220-226
Transport of glycyl- l -leucine into isolated brush border membrane vesicles was studied. On the basis of the following observations it was postulated that glycyl- l -leucine was transported intact by a specific dipeptide mechanism. 1. (1) The differ
Publikováno v:
Journal of Cellular Physiology. 89:805-810
A comparison of L-valine and D-glucose transport was carried out with vesicles of plasma membrane isolated either from the luminal (brush border) or from the contra-luminal (basolateral) region of small intestinal epithelial cells. The existence of t
Autor:
Ulrich Hopfer, Kristine Sigrist-Nelson
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Biomembranes. 367:247-254
The transport of d -fructose in isolated rat intestinal epithelial brush border membranes was studied. 1. 1.|The rates of d -fructose entry appeared to be slower than d -glucose but faster than either l -glucose or d -mannitol. 2. 2.| d -Fructose upt
Characterization of the Dicyclohexylcarbodiimide-Binding Protein Isolated from Chloroplast Membranes
Publikováno v:
European Journal of Biochemistry. 92:9-14
Characterization of a butanol-solubilized protein isolated from chloroplast membranes is reported. The proteolipid, which specifically and covalently binds dicyclohexylcarbodiimide, has an apparent molecular weight of 8,000 in dodecylsulfate electrop
Publikováno v:
Journal of Biological Chemistry. 250:7392-7396
The influence of amino acids on D-glucose transport was studied in isolated vesicles of brush border membrane from rat small intestine. It is demonstrated that: (a) Uptake of D-glucose by the membranes is inhibited by simultaneous flow of L- and D-al
Publikováno v:
FEBS Letters. (1-2):187-191
The sulfoxide Ro 18-5364, a potential metabolite of the IND Ro 18-5362, is a powerful inhibitor of gastric mucosal (H+ + K+)-ATPase, decreasing enzymatic activity with an apparent Ki, of 0.1 μM. Exposure of Ro 18-5364-treated gastric membranes to di
Publikováno v:
FEBS Letters. 95:140-142