Zobrazeno 1 - 5
of 5
pro vyhledávání: '"Kristin Sieg"'
Publikováno v:
American Journal of Transplantation.
Autor:
Martin D. Jendrisak, Kristin Sieg, Kelly Barrios, Bozena Labuda, Andrés Jaramillo, Daniel Magas, Katarzyna Szewczyk
Publikováno v:
Human immunology. 77(6)
Pronase treatment is used in the flow cytometry crossmatch (FCXM) to prevent nonspecific antibody binding on B cells. However, we have observed unexpected positive results with pronase-treated T cells in human immunodeficiency virus (HIV)-infected pa
Autor:
Bozena Labuda, Martin D. Jendrisak, Katarzyna Brooks, Andrés Jaramillo, Justin D. Carrera, Daniel Magas, Kristin Sieg
Publikováno v:
Human Immunology. 75:486
Aim Binding of IgG to FcR on B-cells decreases the sensitivity & specificity of the B-cell FCXM. To prevent this, pronase treatment (PT) of un-separated T- & B-cells is widely used. However, the effect of PT on the T-cell FCXM is not well-documented.
Autor:
Andrés Jaramillo, Katarzyna Brooks, Kristin Sieg, Daniel Magas, Bozena Labuda, Sujata Gaitonde, Martin D. Jendrisak, Amishi Desai
Publikováno v:
American Journal of Clinical Pathology. 140:A078-A078
A positive flow cytometrycrossmatch (FCXM) is often a contraindication for transplantation. Nonspecific binding of IgG antibodies to Fc receptors on B cells decreases sensitivity and specificity of the B-cell crossmatch. To prevent this, pronase trea
Autor:
Martin D. Jendrisak, Andrés Jaramillo, Justin D. Carrera, Daniel Magas, Bozena Labuda, Katarzyna Brooks, Kristin Sieg
Publikováno v:
Human Immunology. 73:66
Aim A positive crossmatch is considered a contraindication for kidney transplantation. Interestingly, we have observed a high rate of positive T-cell FCXM results in the absence of donor-specific antibodies (DSA) in patients infected with human immun