Alteration of Tissue Marking Dyes Depends on Used Chromogen during Immunohistochemistry

Autor: Selina Kiefer, Julia Huber, Hannah Füllgraf, Kristin Sörensen, Agnes Csanadi, Maren Nicole Stillger, Martin Werner, Hans-Eckart Schaefer, Peter Bronsert, Konrad Aumann

Publikováno v: Cells, Vol 10, Iss 4, p 835 (2021)

Pathological biopsy protocols require tissue marking dye (TMD) for orientation. In some cases (e.g., close margin), additional immunohistochemical analyses can be necessary. Therefore, the correlation between the applied TMD during macroscopy and the

Externí odkaz: https://doaj.org/article/73ffb42179874881b8fe408872bcc2fb

Multiple Immunostainings with Different Epitope Retrievals-The FOLGAS Protocol

Autor: Anna von Schoenfeld, Peter Bronsert, Michael Poc, Andrew Fuller, Andrew Filby, Stefan Kraft, Konrad Kurowski, Kristin Sörensen, Julia Huber, Jens Pfeiffer, Michele Proietti, Verena Stehl, Martin Werner, Maximilian Seidl

Publikováno v: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 23, Iss 223, p 223 (2022)
International Journal of Molecular Sciences; Volume 23; Issue 1; Pages: 223

We describe a sequential multistaining protocol for immunohistochemistry, immunofluorescence and CyTOF imaging for formalin-fixed, paraffin-embedded specimens (FFPE) in the formalin gas-phase (FOLGAS), enabling sequential multistaining, independent f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a4578675a2e95fdf94b354dba1d88db
https://pubmed.ncbi.nlm.nih.gov/35008649