Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Kristin M. Slade"'
Publikováno v:
Biochemistry and Biophysics Reports, Vol 26, Iss , Pp 100956- (2021)
In order to better understand how the complex, densely packed, heterogeneous milieu of a cell influences enzyme kinetics, we exposed opposing reactions catalyzed by yeast alcohol dehydrogenase (YADH) to both synthetic and protein crowders ranging fro
Externí odkaz:
https://doaj.org/article/f41d57778b1f4c738631b0bb2a5fa6e7
Autor:
Emily M. Rundlett, Kristin M. Slade
Publikováno v:
The FASEB Journal. 36
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e2406d10ea91019af8cc47ed8d97f500
https://doi.org/10.1096/fasebj.2022.36.s1.r2268
https://doi.org/10.1096/fasebj.2022.36.s1.r2268
Autor:
Kristin M. Slade, Todd P. Silverstein
Publikováno v:
Journal of Chemical Education. 96:2476-2487
Living cells are crowded. The presence of so many macromolecules packed into a confined environment affects the structure and function of biological components, and the kinetics and thermodynamics of biochemical reactions. In fact, crowding studies h
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::97cf2c9b78d42509615186e0887fc656
https://doi.org/10.1021/acs.jchemed.9b00399
https://doi.org/10.1021/acs.jchemed.9b00399
Publikováno v:
Biochemistry and Biophysics Reports
Biochemistry and Biophysics Reports, Vol 26, Iss, Pp 100956-(2021)
Biochemistry and Biophysics Reports, Vol 26, Iss, Pp 100956-(2021)
In order to better understand how the complex, densely packed, heterogeneous milieu of a cell influences enzyme kinetics, we exposed opposing reactions catalyzed by yeast alcohol dehydrogenase (YADH) to both synthetic and protein crowders ranging fro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2774caaf0689491bf55cc2bd7e712c1c
http://europepmc.org/articles/PMC7905371
http://europepmc.org/articles/PMC7905371
Publikováno v:
Biochemistry. 59(18)
Although it is well-known that the environment of mitochondria is a densely packed network of macromolecules, the kinetics of the essential metabolic enzyme, citrate synthase, has been studied only under dilute conditions. To understand how this crow
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8e09332c731576c335522fe623c1dfc5
https://pubmed.ncbi.nlm.nih.gov/32216302
https://pubmed.ncbi.nlm.nih.gov/32216302
Publikováno v:
The FASEB Journal. 33
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::00b714cd2d84d5d03871eddc8680ebdf
https://doi.org/10.1096/fasebj.2019.33.1_supplement.lb217
https://doi.org/10.1096/fasebj.2019.33.1_supplement.lb217
Publikováno v:
Biochemistry. 55:3550-3558
Enzymes operate in a densely packed cellular environment that rarely matches the dilute conditions under which they are studied. To better understand the ramifications of this crowding, the Michaelis-Menten kinetics of yeast alcohol dehydrogenase (YA
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff66c247d7d9023a9fc2bcd5a05f7192
https://doi.org/10.1021/acs.biochem.6b00257
https://doi.org/10.1021/acs.biochem.6b00257
Autor:
Kristin M. Slade, Schuyler P. Lockwood, Micaela A. LoConte, David J. Slade, Bridget E. Logan, Dominique I. Hargreaves, Michael J. Conroy, Samuel H. Schneider, Erin E. McLaughlin
Publikováno v:
Biochemistry. 54:5898-5906
To understand the consequences of macromolecular crowding, studies have largely employed in vitro experiments with synthetic polymers assumed to be both pure and "inert". These polymers alter enzyme kinetics by excluding volume that would otherwise b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a2d550b4b2dfade1f8d019804706bb0
https://doi.org/10.1021/acs.biochem.5b00533
https://doi.org/10.1021/acs.biochem.5b00533
Autor:
Kristin M. Slade, Christopher G. Poggi
Publikováno v:
Biochemistry. 54:260-267
To understand how macromolecular crowding affects enzyme activity, we quantified the Michaelis-Menten kinetics of mitochondrial malate dehydrogenase (MDH) in the presence of hen egg white (HEW), lysozyme, bovine serum albumin (BSA), gum arabic, poly(
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a7f174dbc6d30d2b7d254e0bbf41061
https://doi.org/10.1021/bi5011255
https://doi.org/10.1021/bi5011255
Publikováno v:
Biochemistry. 48:8578-8584
Fibrils of the intrinsically disordered protein alpha-synuclein are hallmarks of Parkinson's disease. The fluorescent dye thioflavin T is often used to characterize fibrillation, but this assay may not provide quantitative information about structure
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3e24160b02e6f90edf82d581c85b3a8
https://doi.org/10.1021/bi900872p
https://doi.org/10.1021/bi900872p