CRISPR-Cas9-directed gene tagging using a single integrase-defective lentiviral vector carrying a transposase-based Cas9 off switch

Autor: Emil Aagaard Thomsen, Kristian Alsbjerg Skipper, Sofie Andersen, Didde Haslund, Thomas Wisbech Skov, Jacob Giehm Mikkelsen

Publikováno v: Molecular Therapy: Nucleic Acids, Vol 29, Iss , Pp 563-576 (2022)

Locus-directed DNA cleavage induced by the CRISPR-Cas9 system triggers DNA repair mechanisms allowing gene repair or targeted insertion of foreign DNA. For gene insertion to be successful, availability of a homologous donor template needs to be timed

Externí odkaz: https://doaj.org/article/3aa9eac12cbf4bfdaa1419c3d3f22f0f

Sustained transgene expression from sleeping beauty DNA transposons containing a core fragment of the HNRPA2B1-CBX3 ubiquitous chromatin opening element (UCOE)

Autor: Kristian Alsbjerg Skipper, Anne Kruse Hollensen, Michael N. Antoniou, Jacob Giehm Mikkelsen

Publikováno v: BMC Biotechnology, Vol 19, Iss 1, Pp 1-11 (2019)

Abstract Background DNA transposon-based vectors are effective nonviral tools for gene therapy and genetic engineering of cells. However, promoter DNA methylation and a near-random integration profile, which can result in transgene integration into h

Externí odkaz: https://doaj.org/article/144b1e9f55574905a37cb7eb483f5a62

Essential role of autophagy in restricting poliovirus infection revealed by identification of an ATG7 defect in a poliomyelitis patient

Autor: Trine H. Mogensen, Kristian Alsbjerg Skipper, Martin K. Thomsen, Ensieh Farahani, Sofie E. Jørgensen, Alon Schneider Hait, Jacob Giehm Mikkelsen, Lise Kay, Søren R. Paludan, Mariane H. Schleimann, Johanna Heinz, Michelle M. Thomsen, Yujia Cai, Nanna-Sophie Brinck Andersen, Simon Muller Larsen

Publikováno v: Brinck Andersen, N S, Jørgensen, S E, Skipper, K A, Larsen, S M, Heinz, J, Thomsen, M M, Farahani, E, Cai, Y, Hait, A S, Kay, L, Giehm Mikkelsen, J, Høgsbjerg Schleimann, M, Thomsen, M K, Paludan, S R & Mogensen, T H 2021, ' Essential role of autophagy in restricting poliovirus infection revealed by identification of an ATG7 defect in a poliomyelitis patient ', Autophagy, vol. 17, no. 9, pp. 2449-2464 . https://doi.org/10.1080/15548627.2020.1831800
Autophagy

Paralytic poliomyelitis is a rare disease manifestation following poliovirus (PV) infection. The disease determinants remain largely unknown. We used whole exome sequencing to uncover possible contributions of host genetics to the development of dise

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52af82cd582d877d97ab3fc590191c99
https://pure.au.dk/portal/da/publications/essential-role-of-autophagy-in-restricting-poliovirus-infection-revealed-by-identification-of-an-atg7-defect-in-a-poliomyelitis-patient(a498e2fb-f7a9-4eb3-8c46-a0a229da79ab).html

Defects in LC3B2 and ATG4A underlie HSV2 meningitis and reveal a critical role for autophagy in antiviral defense in humans

Autor: Søren R. Paludan, Alon Schneider Hait, Sathya Darmalinggam, Mette Christiansen, Simon Muller Larsen, Jørgen Kjems, Darshana D. Kadekar, Kristina Eriksson, Serge Mostowy, Liviu Ionut Moldovan, Nanna-Sophie Brinck Andersen, Vanessa Sancho-Shimizu, Trine H. Mogensen, Lasse Sommer Kristensen, Isobel Parkes, David Olagnier, Marie Helleberg, Demi van der Horst, Michelle M. Thomsen, Mette Ratzer Freytag, Kristian Alsbjerg Skipper, Stine H. Rahbek, Jacob Giehm Mikkelsen, Christian Brandt, Chiranjeevi Bodda, Fanghui Ren

Publikováno v: Hait, A S, Olagnier, D, Sancho-Shimizu, V, Skipper, K A, Helleberg, M, Larsen, S M, Bodda, C, Moldovan, L I, Ren, F, Brinck Andersen, N-S, Thomsen, M M, Freytag, M R, Darmalinggam, S, Parkes, I, Kadekar, D D, Rahbek, S H, van der Horst, D, Kristensen, L S, Eriksson, K, Kjems, J, Mostowy, S, Christiansen, M, Mikkelsen, J G, Brandt, C T, Paludan, S R & Mogensen, T H 2020, ' Defects in LC3B2 and ATG4A underlie HSV2 meningitis and reveal a critical role for autophagy in antiviral defense in humans ', Science Immunology, vol. 5, no. 54, eabc2691 . https://doi.org/10.1126/sciimmunol.abc2691
Hait, A S, Olagnier, D, Sancho-Shimizu, V, Skipper, K A, Helleberg, M, Larsen, S M, Bodda, C, Moldovan, L I, Ren, F, Andersen, N S B, Thomsen, M M, Freytag, M R, Darmalinggam, S, Parkes, I, Kadekar, D D, Rahbek, S H, van der Horst, D, Kristensen, L S, Eriksson, K, Kjems, J, Mostowy, S, Christiansen, M, Mikkelsen, J G, Brandt, C T, Paludan, S R & Mogensen, T H 2021, ' Defects in LC3B2 and ATG4A underlie HSV2 meningitis and reveal a critical role for autophagy in antiviral defense in humans ', Science immunology, vol. 5, no. 54, eabc2691 . https://doi.org/10.1126/SCIIMMUNOL.ABC2691
Science Immunology

Recurrent herpesvirus infections can manifest in different forms of disease, including cold sores, genital herpes, and encephalitis. There is an incomplete understanding of the genetic and immunological factors conferring susceptibility to recurrent

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76e2d27ad827935f3a3c72318f2fa311
https://pure.au.dk/portal/da/publications/defects-in-lc3b2-and-atg4a-underlie-hsv2-meningitis-and-reveal-a-critical-role-for-autophagy-in-antiviral-defense-in-humans(660e28c6-e88f-4155-8813-69748e66d63d).html

Toward In Vivo Gene Therapy Using CRISPR

Autor: Kristian Alsbjerg, Skipper, Jacob Giehm, Mikkelsen

Publikováno v: Methods in molecular biology (Clifton, N.J.). 1961

CRISPR, a revolutionizing technology allowing researchers to navigate in and edit the genome, is moving on the fast track toward clinical use for ex vivo correction of disease-causing mutations in stem cells. As we await the first trials utilizing ex

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::ecc6b3e223363b9a5e97df38c5b59ac5
https://pubmed.ncbi.nlm.nih.gov/30912053