Evidence for a role of spindle matrix formation in cell cycle progression by antibody perturbation.

Autor: Changfu Yao, Chao Wang, Yeran Li, Michael Zavortink, Vincent Archambault, Jack Girton, Kristen M Johansen, Jørgen Johansen

Publikováno v: PLoS ONE, Vol 13, Iss 11, p e0208022 (2018)

In Drosophila it has recently been demonstrated that a spindle matrix in the form of a membrane-less macromolecular assembly embeds the microtubule-based spindle apparatus. In addition, two of its constituents, Megator and Chromator, were shown to fu

Externí odkaz: https://doaj.org/article/163badb7e2994f72859f0510bc480889

The spindle matrix protein, Chromator, is a novel tubulin binding protein that can interact with both microtubules and free tubulin.

Autor: Changfu Yao, Chao Wang, Yeran Li, Yun Ding, Uttama Rath, Saheli Sengupta, Jack Girton, Kristen M Johansen, Jørgen Johansen

Publikováno v: PLoS ONE, Vol 9, Iss 7, p e103855 (2014)

The chromodomain protein, Chromator, is localized to chromosomes during interphase; however, during cell division together with other nuclear proteins Chromator redistributes to form a macro molecular spindle matrix complex that embeds the microtubul

Externí odkaz: https://doaj.org/article/5e24be7210d4479eae21f8391b15bb41

Phosphorylation of SU(VAR)3-9 by the chromosomal kinase JIL-1.

Autor: Joern Boeke, Catherine Regnard, Weili Cai, Jørgen Johansen, Kristen M Johansen, Peter B Becker, Axel Imhof

Publikováno v: PLoS ONE, Vol 5, Iss 4, p e10042 (2010)

The histone methyltransferase SU(VAR)3-9 plays an important role in the formation of heterochromatin within the eukaryotic nucleus. Several studies have shown that the formation of condensed chromatin is highly regulated during development, suggestin

Externí odkaz: https://doaj.org/article/ab240705a92e488985f7c591547372b9

JASPer controls interphase histone H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila

Autor: Tom W. Muir, Peter B. Becker, Sylvain Maenner, Jørgen Johansen, Yeran Li, Catherine Regnard, Jack Girton, Kristen M. Johansen, Felix Wojcik, Tamas Schauer, Christian Albig, Geoffrey P. Dann, Weili Cai, Silke Krause, Chao Wang

Publikováno v: Nature Communications
Nature Communications, Nature Publishing Group, 2019, 10 (1), pp.5343. ⟨10.1038/s41467-019-13174-6⟩
Nature Communications, Vol 10, Iss 1, Pp 1-17 (2019)

In flies, the chromosomal kinase JIL-1 is responsible for most interphase histone H3S10 phosphorylation and has been proposed to protect active chromatin from acquiring heterochromatic marks, such as dimethylated histone H3K9 (H3K9me2) and HP1. Here,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::387d041d769ad214d4ca0e1dadf53e13
https://hal.univ-lorraine.fr/hal-03212086

Movement of chromosomes with severed kinetochore microtubules

Autor: Kristen M. Johansen, Arthur Forer, Jørgen Johansen

Publikováno v: Protoplasma. 252:775-781

Experiments dating from 1966 and thereafter showed that anaphase chromosomes continued to move poleward after their kinetochore microtubules were severed by ultraviolet microbeam irradiation. These observations were initially met with scepticism as t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c8ca0d9e18cfe17e8276e62ac47443cb
https://doi.org/10.1007/s00709-014-0752-7